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PF073 TGF-β3, Human, Recombinant, S. frugiperda

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Transforming Growth Factor-β3

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PF073
  
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Overview

Replacement Information

Key Spec Table

Purity
≥95% by SDS-PAGE
Description
Overview

This product has been discontinued.



Recombinant, human transforming growth factor-β3 expressed in S. frugiperda insect cells.
Catalogue NumberPF073
Brand Family Calbiochem®
SynonymsTransforming Growth Factor-β3
References
ReferencesMehler, M.F. and Kessler, J.A. 1995. Crit. Rev. Neurobiol. 9, 419.
Burton, P.B.J., et al. 1993. Int. J. Expl. Pathol. 74, 87.
Massague, J., et al. 1992. Cell 69, 1067.
Sporn, M.B. and Roberts, A.B. 1992. J. Cell Biol. 119, 1017.
Kondaiah, P., et al. 1990. J. Biol. Chem. 265, 1089.
Massague, J. 1990. Ann. Rev. Cell Biol. 6, 597.
Roberts, A.B. and Sporn, M.B. 1990. In Peptide Growth Factors and Their Receptors, ed. M. Sporn, A.B. Roberts. Heidelberg: Springer-Verlag.
Tsang, M., et al. 1990. Lymphokine Res. 9, 607.
Lyons, R.M., et al. 1988. J. Cell Biol. 106, 1659.
Madisen, L., et al. 1988. DNA 7, 1.
Ten Dijke, P., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 4715.
Derynck, R., et al. 1987. Nucleic Acids Res. 15, 3188.
Massague, J. 1987. Cell 49, 437.
Product Information
FormLyophilized
FormulationLyophilized from 30% acetonitrile, 0.1% TFA, 50 µg BSA/µg of cytokine.
PreservativeNone
Quality LevelMQ100
Applications
Biological Information
Biological activityEC₅₀ = 0.01-0.03 ng/ml as determined by the ability to inhibit IL-4-dependent ³H-thymidine incorporation in mouse HT-2 cells.
Purity≥95% by SDS-PAGE
Physicochemical Information
ContaminantsEndotoxin: ≤1.0 EU/µg cytokine
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. Purified human recombinant TGF-β3 is an extremely hydrophobic protein that adheres strongly to surfaces.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
PF073 0

Documentation

TGF-β3, Human, Recombinant, S. frugiperda SDS

Title

Safety Data Sheet (SDS) 

TGF-β3, Human, Recombinant, S. frugiperda Certificates of Analysis

TitleLot Number
PF073

References

Reference overview
Mehler, M.F. and Kessler, J.A. 1995. Crit. Rev. Neurobiol. 9, 419.
Burton, P.B.J., et al. 1993. Int. J. Expl. Pathol. 74, 87.
Massague, J., et al. 1992. Cell 69, 1067.
Sporn, M.B. and Roberts, A.B. 1992. J. Cell Biol. 119, 1017.
Kondaiah, P., et al. 1990. J. Biol. Chem. 265, 1089.
Massague, J. 1990. Ann. Rev. Cell Biol. 6, 597.
Roberts, A.B. and Sporn, M.B. 1990. In Peptide Growth Factors and Their Receptors, ed. M. Sporn, A.B. Roberts. Heidelberg: Springer-Verlag.
Tsang, M., et al. 1990. Lymphokine Res. 9, 607.
Lyons, R.M., et al. 1988. J. Cell Biol. 106, 1659.
Madisen, L., et al. 1988. DNA 7, 1.
Ten Dijke, P., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 4715.
Derynck, R., et al. 1987. Nucleic Acids Res. 15, 3188.
Massague, J. 1987. Cell 49, 437.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision16-September-2008 RFH
SynonymsTransforming Growth Factor-β3
DescriptionRecombinant, human transforming growth factor-β3 expressed in S. frugiperda insect cells. The disulfide-linked homodimeric human recombinant TGF-β3 contains two 112 amino acid peptides and has a predicted molecular weight of ~25 kDa. Useful for growth ihibition assays and other assays designed to study cellular responses and receptor interactions involving TGF. The transforming growth factor (TGF) superfamily contains structurally related secretory peptides, which become activated by proteolytic cleavage. In addition to the TGF-βs, other family members include the activins, inhibins, bone morphogenetic proteins (BMPs), glial cell-line derived neurotrophic factor (GDNF), and Mullerian inhibitory substance (MIS). The three TGF-β isoforms, TGF-β1, TGF-β2, and TGF-β3, are potent regulators of cell growth and differentiation, extracellular matrix formation, and inflammatory events. The TGF-βs bind to three high affinity serine/threonine kinase receptors, designated type I (~55-65 kDa), type II (~70-85 kDa), and type III/betaglycan (~200-400 kDa). Structurally, the TGF-β1, β2, and β3 proteins are homodimers composed of disulfide linked chains 112 amino acids in length. Composition of TGF-β proteins from different species is highly conserved at the DNA level as well as at the protein level showing in general >85% identity within a subtype and 64-82% identity between subtypes. TGF-β1 and β2 were first identified on the basis of their biological activity and subsequently cloned from expression libraries. Human TGF-β3 was identified first at the cDNA level and of the three major isoforms, appears to be the most biologically active. The expression of TGF-β3 at various stages of morphogenesis and at different sites suggests an important role in the developmental process.
FormLyophilized
FormulationLyophilized from 30% acetonitrile, 0.1% TFA, 50 µg BSA/µg of cytokine.
Purity≥95% by SDS-PAGE
ContaminantsEndotoxin: ≤1.0 EU/µg cytokine
Biological activityEC₅₀ = 0.01-0.03 ng/ml as determined by the ability to inhibit IL-4-dependent ³H-thymidine incorporation in mouse HT-2 cells.
SolubilityReconstitute in sterile 4 mM HCl containing at least 0.1% human serum albumin or bovine serum albumin to a final concentration of ≥1 µg/ml.
PreservativeNone
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. Purified human recombinant TGF-β3 is an extremely hydrophobic protein that adheres strongly to surfaces.
Toxicity Standard Handling
ReferencesMehler, M.F. and Kessler, J.A. 1995. Crit. Rev. Neurobiol. 9, 419.
Burton, P.B.J., et al. 1993. Int. J. Expl. Pathol. 74, 87.
Massague, J., et al. 1992. Cell 69, 1067.
Sporn, M.B. and Roberts, A.B. 1992. J. Cell Biol. 119, 1017.
Kondaiah, P., et al. 1990. J. Biol. Chem. 265, 1089.
Massague, J. 1990. Ann. Rev. Cell Biol. 6, 597.
Roberts, A.B. and Sporn, M.B. 1990. In Peptide Growth Factors and Their Receptors, ed. M. Sporn, A.B. Roberts. Heidelberg: Springer-Verlag.
Tsang, M., et al. 1990. Lymphokine Res. 9, 607.
Lyons, R.M., et al. 1988. J. Cell Biol. 106, 1659.
Madisen, L., et al. 1988. DNA 7, 1.
Ten Dijke, P., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 4715.
Derynck, R., et al. 1987. Nucleic Acids Res. 15, 3188.
Massague, J. 1987. Cell 49, 437.