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69999 T7•Tag® Antibody AP Conjugate

T7•Tag® Antibody AP Conjugate MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
69999
  
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Overview

Replacement Information
Description
Overview

This product has been discontinued.



The T7•Tag AP Conjugate is useful as a single detection reagent and eliminates the need for second antibodies or streptavidin conjugates. The enzyme conjugated T7•Tag monoclonal antibody enables detection with only one binding step and eliminates cross-reactivity associated with secondary reagents.

The T7•Tag® Antibody is a mouse monoclonal antibody (IgG2b) directed against the 11 amino acid gene 10 leader peptide expressed by many of the pET translation vectors as well as pSCREEN™ and pRSET vectors. Because the peptide is the natural amino terminal end of the T7 major capsid protein, the antibody also recognizes T7 bacteriophage (e.g., the T7Select™ Vectors).

This antibody is also available labeled with biotin and as a direct conjugate with horseradish peroxidase.

SpecificityT7•Tag peptide MetAlaSerMetThrGlyGlyGlnGlnMetGly; N-terminal, C-terminal or internal
Species/IsotypeMouse monoclonal IgG2b
Cross-reactivityNegligible with bacterial, insect or mammalian cell lysates
Sensitivity1–10 ng (Western blot developed with chromogenic substrates)
< 1 ng (developed with chemiluminescent substrates)
FormStabilized solution of antibody conjugate in 50% glycerol; includes Positive Control Extract containing 31.1 kDa target protein
Working dilution1:5,000–1:10,000
Catalogue Number69999
Brand Family Novagen®
References
Product Information
Components
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
69999 0

Documentation

T7•Tag® Antibody AP Conjugate Certificates of Analysis

TitleLot Number
69999

Citations

Title
  • Nadia L. Korneeva, et al. (2005) Interaction between the NH2-terminal domain of eIF4A and the central domain of eIF4G modulates RNA-stimulated ATPase activity. Journal of Biological Chemistry (280) 1872-1881.
  • S. Dean Rider, et al. (2005) The protozoan parasite Cryptosporidium parvum possesses two functionally and evolutionarily divergent replication protein A large subunits. Journal of Biological Chemistry 280, 31460-31469.
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    User Protocols

    Title
    TB112 T7•Tag® Antibody AP Conjugate