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69963 Straight A's™ mRNA Isolation System

Straight A's™ mRNA Isolation System MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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Overview

Replacement Information
Description
Overview

This product has been discontinued.





The Straight A's™ mRNA Isolation System is designed for rapid isolation of high quality poly(A)+ RNA from tissue without using organic extraction (McCormick 1994). The key component of the kit is the Magnetight™ Oligo(dT) Particles, which are encapsulated paramagnetic particles that contain covalently attached oligo(dT)25 to directly bind mRNA. The coated magnetic particles have been treated to minimize non-specific interactions and enable quantitative recovery of intact mRNA. The entire procedure requires less than one hour and is applicable to fresh or frozen tissue. Using the Straight A's System, high quality mRNA can be isolated from tissue, cultured cells, or total RNA, and the procedure can be easily scaled from micro scale to grams of tissue. Because ribonuclease activity is effectively inhibited during tissue lysis, several samples can be processed in parallel without degradation of mRNA.

The Straight A's mRNA Isolation System provides sufficient components to isolate poly(A)+ RNA from up to 2 g animal tissue, 8 g plant tissue, or 4 × 108 cultured animal cells. The Introductory System provides components to process 1/10 of these amounts.
Catalogue Number69963
Brand Family Novagen®
Features and benefits
  • Isolate poly(A)+ RNA directly from tissue in less than 1 hour
  • Purity > 75% poly(A)+ RNA after one round, > 95% after two rounds
  • Intact mRNA ready for any application: RT-PCR, cDNA synthesis, in vitro translation, etc.
  • Requires no phenol, guanidine, or chloroform
  • Minimal centrifugation: rapid magnetic separations (< 1 minute)
  • System works with animal tissue, plant tissue, or cultured cells
  • Suitable for second round isolation and poly(A)+ selection from total RNA
  • Usable at any scale: milligrams to grams of tissue
  • Reusable Magnetight™ Oligo(dT) Particles
  • Reasonably priced magnetic particles and separation stands
References
ReferencesMcCormick, M. and Hammer, B. 1994. inNovations 2, 8.
Product Information
Components
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage Multiple storage requirements
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
69963 0

Documentation

Straight A's™ mRNA Isolation System Certificates of Analysis

TitleLot Number
69963

References

Reference overview
McCormick, M. and Hammer, B. 1994. inNovations 2, 8.

Citations

Title
  • Norimoto Shimada, et al. (2007) Genome-wide analyses of the structural gene families involved in the legume-specific 5-deoxyisoflavonoid biosynthesis of Lotus japonicus. DNA Research 14, 25-36.
  • Bryan M. Wittmann, Andrea Sherk and Donald P. McDonnell. (2007) Definition of functionally important mechanistic differences among selective estrogen receptor down-regulators. Cancer Research 67, 9549-9560.
  • Hirokazu Suzuki, et al. (2006) An isoflavone conjugate-hydrolyzing beta-glucosidase from the roots of soybean (Glycine max) seedlings: purification, gene cloning, phylogenetics, and cellular localization. Journal of Biological Chemistry 281, 30251-30259.
  • Shin'ya Sawada, et al. (2005) UDP-glucuronic acid: anthocyanin glucuronosyltransferase from red daisy (Bellis perennis) flowers: enzymology and phylogenetics of a novel glucoronosyl transferase involved in flower pigment biosynthesis. Journal of Biological Chemistry 280, 899-906.
  • Norimoto Shimada, et al. (2003) A cluster of genes encodes the two types of chalcone iIsomerase involved in the biosynthesis of general flavonoids and legume-specific 5-deoxy(iso)flavonoids in Lotus japonicus. Plant Physiology 131, 941-951.
  • Sylvie Baudino, et al. (2001) Molecular characterisation of two novel maize LRR receptor-like kinases, which belong to the SERK gene family. Planta 213, 1-10.
  • Richard S. Masser, Robert Zinkel and John H.J. Petrini. (2001) An alternative mode of translation permits production of a variant NBS1 protein from the common Nijmegen breakage syndrome allele. Nature Genetics 27, 417-421.
  • Krista L. Thomas and K. Peter Pauls. (2001) A method of subtractive hybridization mediated by oligo(dT) particles. Plant Molecular Biology Reporter 19, 373a-373f.
  • Sydney Brenner, et al. (2000) Gene expression analysis by massively parallel signature sequencing (MPSS) on microbead arrays. Nature Biotechnology 18, 630-634.
  • Norimoto Shimada, et al. (2000) Induction of isoflavinoid pathway in the model legume Lotus japonicus: molecular characterization of enzymes involved in phytoalexin biosynthesis. Plant Science 160, 37-47.
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    User Protocols

    Title
    TB103