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Stericup® Filter Units

The Stericup vacuum filtration system combines a Steritop vacuum filtration unit with a receiver flask for processing and storing volumes from 150 mL to 1,000 mL.

The Stericup vacuum filtration system combines a Steritop filter unit with a receiver flask for processing and storing volumes from 150 mL to 1,000 mL.

Its new no tip/easy grip flask design and compact profile improve stability during filtration, make gripping the receiver easier, and make Stericup Filter Units ideal for use in laminar flow hoods.

As an added convenience, the bottom of the receiver flask is slightly recessed, enabling capped flasks to be stacked for convenient storage.

Overview

Specifications

Ordering Information

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Units with fast flow and low protein binding 0.1 µm Millipore Express (PES) membrane for sterilization of aqueous solutions and mycoplasma removal; supplied sterileClear Sorting & Filtering Show Filter
Catalogue NumberDescriptionFunnel CapacityReceiverPack Size
SCVPU02REStericup-VP Sterile Vacuum Filtration System 250 mL 250 mL bottle with cap 12 pack, VP, 0.10 µm, polyethersulfone, 250 mL, radio-sterilized

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Units with fast flow and low protein binding 0.22 µm Millipore Express PLUS (PES) membrane for sterilization of aqueous solutions; supplied sterile*Clear Sorting & Filtering Show Filter
Catalogue NumberDescriptionFunnel CapacityReceiverPack Size
SCGPU01REStericup-GP Sterile Vacuum Filtration System 150 mL 150 mL bottle with cap 12 pack, GP, 0.22 µm, polyethersulfone, 150 mL, radio-sterilized

* These products have been tested for use in stem cell research applications. To determine their effects on Mouse Stem Cell growth or differentiation, three lots of Stericup-GP devices were used to filter media with LIF. Once filtered, this media was used to passage mouse stem cells five times to verify that Stericup-GP filtration does not impact pluripotency of mouse stem cells.

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Units with very low protein binding 0.22 µm Durapore (PVDF) membrane for sterilization of aqueous solutions; supplied sterileClear Sorting & Filtering Show Filter
Catalogue NumberDescriptionFunnel CapacityReceiverPack Size
SCGVU05REStericup-GV Sterile Vacuum Filtration System 500 mL 500 mL bottle with cap 12

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Documentation

FAQ

QuestionAnswer
Are Stericup and Steritop units autoclavable?No. The highest temperature that the product can be used at is 50C ( 122F ).
Can I filter 100% serum with the Stericup?Yes. The Express membrane is the highest throughput membrane available on the market today.
Can I purchase extra receiver flasks for the Stericups separately?Yes, the catalog number is SC00B10RE.
Can you freeze Stericup units?You can successfully freeze and store many aqueous solutions ( such as culture media ) at temperatures to -20C ( -4F ). We strongly recommend that you run a trial under actual conditions to test for suitability of the bottles for frozen storage.
Can I bubble point my Stericup/top?No. The unit is not designed to handle the pressures required to perform a bubble point test. It is designed to be run under vacuum only (15 psi max). Bubble points for any of the membranes in the Stericups/tops need to be run at pressures ranging from 22 psi to 57psi.
Do you have a filter for 100% serum?Yes, you can filter serum with Millipore Express membrane. The Express membrane was designed for this application and offers fast filtration time. Millipore Express is available in a large number of filter devices including Millex syringe filters, Stericup and Steritop vacuum devices, Sterivac for large volumes of media and Steriflip for 50 mls.
For tissue culture media filtration which membrane should I use?The best membrane for this application is the Millipore Express membrane. It was designed specifically to filter tissue culture media with serum. It is low protein binding, has fast flow and high throughput (which is also called filter capacity).
I want to sterilize 100% ethanol. Are any of the Stericups or Steritops compatible with ethanol?The funnel and receiver flask is made of polystyrene. Any molded polystyrene will craze in pure ethanol and could structurally fail depending on the forces being applied to it. The funnel could handle the short exposure time but the receiver would craze eventually. Therefore we would not recommend the Stericup with 100% ethanol. The Steritop, if you keep the exposure time to the funnel minimal, should be fine. As you dilute the alcohol the compatibility improves.

Both Express and Durapore membranes are compatible. MCE is not.
I will be filtering tissue culture media with your Stericup/top and am concerned that wetting agents in the filter could effect my cells. Which membrane type would you recommend to avoid this?All of Millipore's Tissue Culture Media filtration sterilization devices contain membranes that are wetting agent (surfactant ) free.
Is the Express membrane surfactant free?Yes, the Express membrane is surfactant free. It also has a very low extractables specification - less then 1.5% by weight.

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Categories

Life Science Research > Cell Culture and Systems > Sterile Filtration Devices