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SCC287 SQ-20B Human Squamous Cell Carcinoma Cell Line

SQ-20B human squamous cell carcinoma cell line is a widely used model for radiation-resistant HNSCC.

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SCC287
≥1X10⁶ cells/vial   
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Overview

Replacement Information
Description
Catalogue NumberSCC287
DescriptionSQ-20B Human Squamous Cell Carcinoma Cell Line
Alternate Names
  • SQ20B
  • SQ20-B
Background InformationSquamous cell carcinoma of the head and neck (HNSCC) represents 90% of all head and neck cancers (1). HNSCC is the 9th-most common cancer worldwide and is characterized by a high rate of recurrence after therapy, with a median 5-year survival range of 40-50% (1). Radiation resistance can arise when cells undergo sublethal damage during x-ray treatment, giving rise refractory post-treatment tumors with poor prognosis (2). Cellular models of HNSCC that demonstrate radiation-resistance are critical for elucidating factor associated with radio-resistance and advancing effective therapies.

The SQ-20B human laryngeal squamous cell carcinoma cell line is a widely used model for radiation-resistant HNSCC. SQ-20B cells were derived from a laryngeal tumor (tumor stage 2 node stage 0) that presented following primary radiation therapy (2). The SQ-20B cell line harbors the c-raf proto-oncogene (3) associated with tumorigenicity and radio-resistance (4). SQ-20B cells have been characterized by expression of the SCC marker p63 (p40) (5). The characteristics of the SQ-20B cell line demonstrate its utility as a model for cellular mechanisms that give rise to radiation resistance.

Source:
The SQ-20B cell line was derived from a tumor on the larynx of a patient undergoing radiation therapy2 and harbors a male karyotype (3).

References:
1. J Immunother Cancer. 2019; 7(1): 184.
2. Proc Natl Acad Sci USA. 1986; 83(8): 2684-8.
3. Science. 1987; 237(4818): 1039-41.
4. Science. 1989; 243(4896): 1354-6.
5. Diagn Cytopathol. 2009; 37(3): 178-83.
References
Product Information
Applications
ApplicationSQ-20B human squamous cell carcinoma cell line is a widely used model for radiation-resistant HNSCC.
Key Applications
  • Cell Culture
Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
Biological Information
Cell Line Type
  • Cancer Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance• Each vial contains ≥ 1X10⁶ viable cells.
• Cells are tested negative for infectious diseases by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are verified to be of human origin and negative for inter-species contamination from rat, mouse, chinese hamster, Golden Syrian hamster, and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination.
• Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Packaging Information
Material Size≥1X10⁶ cells/vial
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
SCC287 04061842104079