Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

03-0134-00 SMC™ Mouse IL-10 Discovery Immunoassay Kit

The sensitivity of Single Molecule Counting (SMC™) technology gives the SMC™ Mouse IL-10 Discovery Immunoassay has unparalleled performance in quantifying IL-10 in mouse serum.

SMC™ Mouse IL-10 Discovery Immunoassay Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Single Molecule Counting (SMC®) Research Category: Inflammation & Immunology Research Sub-Category: Cytokines & Cytokine Receptors
View Products on Sigmaaldrich.com
03-0134-00
96 well assay  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Analytes AvailableSpecies ReactivityKey Applications
IL-10 MSingle Molecule Counting (SMC®)
Description
Catalogue Number03-0134-00
Trade Name
  • SMC®
DescriptionSMC™ Mouse IL-10 Discovery Immunoassay Kit
OverviewThe SMC™ Mouse IL-10 Discovery Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC™ immunoassay system detects analytes using Single Molecule Counting (SMC™) technology.
Background InformationInterleukin-10 (IL-10) is a cytokine with both stimulatory and suppressive effects on the immune system. The protein originates from several cell lines, including Th2 cells, mast cells, macrophages, monocytes and B-cells. Its main functions are to impede inflammatory responses and to inhibit synthesis of other pro-inflammatory cytokines, such as IL-2, IL-3, GM-CSF, IFN-γ and TNF-α. Besides its anti-inflammatory capabilities, IL-10 also regulates the production of natural killer cells and lymphocytes. Due to its multiple roles, proper function of IL-10 is required to maintain a healthy immune system. Improper function of IL-10 can result in disease development, as over expression of IL-10 has been linked to lymphoma, whereas IL-10 deficiency has implications for Crohn’s disease.
References
Product Information
Components
  • Assay Buffer
  • Mouse IL-10 Coating Antibody
  • Assay Plate
  • 10X PBS
  • Blocking Buffer
  • Standard Diluent
  • Mouse IL-10 Detection Antibody
  • Mouse IL-10 Standard
  • SMC™ Mouse IL-10 Immunoassay Kit Instructions
  • 10X Wash Buffer
  • Buffer C
  • Elution Buffer B
Precision, %
  • Low-end CV% Range: 0-11% Low-end CV% Average: 6%
Quality LevelMQ200
Applications
ApplicationThe sensitivity of Single Molecule Counting (SMC™) technology gives the SMC™ Mouse IL-10 Discovery Immunoassay has unparalleled performance in quantifying IL-10 in mouse serum.
Key Applications
  • Single Molecule Counting (SMC®)
Biological Information
Species Reactivity
  • Mouse
Analytes Available
  • IL-10
Accuracy
  • Minimum of 10 samples spiked with acceptable recovery between 80-120%.
Physicochemical Information
Linearity of DilutionMinimum of 10 samples spiked and diluted (at least 3 dilutions) with acceptable recovery between 80-120%.
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size96 well assay
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
03-0134-00 04055977317749