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03-0153-00 SMC™ Glucagon High Sensitivity Immunoassay Kit

The sensitivity of the Single Molecule Counting (SMC™) technology gives the SMC™ Glucagon High Sensitivity Immunoassay Kit unparalleled performance in the quantification of Glucagon in human, mouse an

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Key Applications: Single Molecule Counting (SMC®) Research Category: Metabolism Research Sub-Category: Obesity, Metabolic Disorders
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03-0153-00
96 well assay  
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Overview

Key Spec Table

Key Applications
Single Molecule Counting (SMC®)
Description
Catalogue Number03-0153-00
Trade Name
  • SMC®
DescriptionSMC™ Glucagon High Sensitivity Immunoassay Kit
OverviewThe SMC™ Glucagon High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The Erenna® and SMCxPRO™ immunoassay systems detect analytes using Single Molecule Counting (SMC™) technology.
Background InformationGlucagon is the most important counter-regulatory hormone to insulin in blood glucose homeostasis. It elevates blood glucose levels by stimulating gluconeogenesis and glycogenolysis while inhibiting glycogenesis and gylcolysis in the liver. This hormone is extremely important in studying therapeutic targets for diabetes, particularly Type I.
Product Information
Components
  • Standard Diluent
  • Glucagon Standard
  • Assay Buffer
  • Glucagon Beads
  • Glucagon Detection
  • 10X Wash Buffer
  • Buffer D
  • Elution Buffer B
Precision, %
  • Intra-assay CV% average (n=20): <10%
  • Inter-assay CV% average (n=6): <15%
Applications
ApplicationThe sensitivity of the Single Molecule Counting (SMC™) technology gives the SMC™ Glucagon High Sensitivity Immunoassay Kit unparalleled performance in the quantification of Glucagon in human, mouse and rat plasma.
Key Applications
  • Single Molecule Counting (SMC®)
Biological Information
Cross ReactivityNo significant cross-reactivity to related proteins was observed (<0.5% to human Oxyntomodulin and 0% to human GLP-1, GLP-2, and GIP).
Accuracy
  • Minimum of 5 samples spiked with acceptable recovery between 80-120%.
Physicochemical Information
Sensitivity
  • Lower Limit of Quantification: 0.781 pg/mL
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Packaging Information
Material Size96 well assay
Global Trade Item Number
Catalogue Number GTIN
03-0153-00 04054839455797