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69060 S-Protein FITC Conjugate

69060
  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Overview

This product has been discontinued.





The S-protein FITC Conjugate is designed for in situ fluorescent detection of S•Tag™ fusion proteins. It has been optimized for maximum signal without loss in S-protein binding activity. Because of its small size, this conjugate penetrates cells rapidly. This makes it ideal for staining insect or mammalian cells to confirm recombinant protein expression. The 200 µl size will stain a minimum of 200 slides. The recommended working dilution for cell staining is 1:2,000.
Catalogue Number69060
Brand Family Novagen®
Application Data
S-protein FITC detection of baculovirus-expressed S·Tag fusion protein in infected Sf9 cells A BacVector® recombinant baculovirus expressing an S·Tag fusion protein was used to infect Ready-Plaque Sf9 Cells. Forty-eight hours after infection, the cells were processed and stained with S-protein FITC. Left panel, fluorescent illumination; right panel, phase contrast photo of the same field. Lower right insets are uninfected control cells. The Western blot insert in the right panel was performed with a total cell extract loaded in parallel with Novagen's Perfect Protein™ Markers and developed with S-protein AP Conjugate, which recognizes both the S·Tag fusion protein product and the markers.
References
Product Information
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
69060 0

Documentation

S-Protein FITC Conjugate SDS

Title

Safety Data Sheet (SDS) 

S-Protein FITC Conjugate Certificates of Analysis

TitleLot Number
69060

Citations

Title
  • Malla Padidam, Roger N. Beachy and Claude E. Fauquet. (1999) A phage single-stranded DNA (ssDNA) binding protein complements ssDNA accumulation of a geminivirus and interferes with viral movement. Journal of Virology 73, 1609-1616.
  • N.C. Chi and S.A. Adam. (1997) Functional domains in nuclear import factor p97 for binding the nuclear localization sequence receptor and the nuclear pore. Molecular Biology of the Cell 8, 945-956.
  • User Protocols

    Title
    TB143