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HTS093RTA Ready-to-Assay™ FP Prostanoid Receptor Frozen Cells

Human FP GPCR Frozen Cells for calcium flux FLIPR Assays.

Host Cell: Chem-1
HTS093RTA
1 kit  
Purchase on Sigma-Aldrich

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Overview

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This product requires a User Agreement (Label License). Please see product datasheet for further information

Key Spec Table

SpeciesHost CellsProtein Target
Human Chem-1FP
Description
Catalogue NumberHTS093RTA
Trade Name
  • Ready-to-Assay
DescriptionReady-to-Assay™ FP Prostanoid Receptor Frozen Cells
Background InformationMillipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

Prostanoids are a series of arachidonic acid metabolites produced by the action of cyclooxygenase and subsequently by isomerases and synthases. Cells rapidly secrete prostanoids after synthesis, whereupon the prostanoids bind to a family of 8 GPCRs to exert their biological effects (Narumiya and FitzGerald, 2001). The prostaglandin PGF2alpha binds specifically to the FP receptor, which couples to Gq/11 to mobilize intracellular calcium. Binding of PGF2alpha to FP receptors in the corpus luteum is required for luteolysis and subsequent parturition in mice (Sugimoto et al., 1998). PGF2alpha also decreases intraocular pressure by an FP-dependent mechanism, and an PGF2--analog--latanoprost, is used clinically in the treatment of glaucoma (Crowston et al., 2004). FP also contributes to tachycardia induced by inflammatory stimuli (Takayama et al., 2005). Chemicon's cloned human FP-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant FP expression on the cell surface and contains high levels of the promiscuous G protein Galpha15 to enhance coupling of the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between FP and its ligands.
Product Information
Components
  • Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
  • Fifty (50) mL of Media Component.
Quality LevelMQ300
Applications
ApplicationHuman FP GPCR Frozen Cells for calcium flux FLIPR Assays.
Key Applications
  • Ligand Binding Assays
Biological Information
GPCR ClassA
Host CellsChem-1
Protein TargetFP
Target Sub-FamilyProstanoid
Cell Line Type
  • GPCR Cell Lines
Product Usage Statements
Sales RestrictionsThis product requires a User Agreement (Label License). Please see product datasheet for further information.
Usage Statement
  • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsPlace cells in liquid nitrogen immediately upon receipt.
Packaging Information
Material Size1 kit
Global Trade Item Number
Catalogue Number GTIN
HTS093RTA 04053252629877