Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

559270 Rb/Raf-1 Disruptor 251

A cell-permeable and orally active isothiouronium compound that potently and selectively disrupts the Rb/Raf-1 interaction, while exhibiting no effect against Rb/B-Raf, Rb/E2F1, Rb/HDAC1, Raf-1/MEK1.

Rb/Raf-1 Disruptor 251 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: S-(2,4-Dichlorobenzyl)-isothiouronium chloride, RRD-251

View Products on Sigmaaldrich.com
559270
  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
72214-67-6C₈H₉Cl₃N₂S
Description
OverviewA cell-permeable and orally active isothiouronium compound that potently and selectively disrupts the Rb/Raf-1 interaction (IC50 = 77 nM in cell-free binding assays), while exhibiting no effect against Rb/B-Raf, Rb/E2F1, Rb/prohibitin, Rb/cyclin E, Rb/HDAC1, Raf-1/MEK1, or Raf-1/MEK2 interaction. Suppresses cellular E2F transcription activity and reduces serum-induced Rb phosphorylation. Shown to display Rb-dependent antitumor activity both in cultures in vitro and in mice in vivo.
Catalogue Number559270
Brand Family Calbiochem®
SynonymsS-(2,4-Dichlorobenzyl)-isothiouronium chloride, RRD-251
References
ReferencesKinkade, R., et al. 2008. Cancer Res. 68, 3810.
Product Information
CAS number72214-67-6
FormWhite solid
Hill FormulaC₈H₉Cl₃N₂S
Chemical formulaC₈H₉Cl₃N₂S
Hygroscopic Hygroscopic
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Purity≥97% by HPLC
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 36/37/38

Irritating to eyes, respiratory system and skin.
S PhraseS: 26-36-45

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Irritant
Storage +2°C to +8°C
Protect from Light Protect from light
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C. However, it is strongly recommended that stock solutions be prepared fresh just prior to each use.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
559270 0

Documentation

Rb/Raf-1 Disruptor 251 SDS

Title

Safety Data Sheet (SDS) 

Rb/Raf-1 Disruptor 251 Certificates of Analysis

TitleLot Number
559270

References

Reference overview
Kinkade, R., et al. 2008. Cancer Res. 68, 3810.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision19-April-2011 RFH
SynonymsS-(2,4-Dichlorobenzyl)-isothiouronium chloride, RRD-251
DescriptionA cell-permeable and orally active isothiouronium compound that potently and selectively disrupts the Rb/Raf-1 interaction (IC50 = 77 nM in cell-free binding assays), while exhibiting no effect against Rb/B-Raf, Rb/E2F1, Rb/prohibitin, Rb/cyclin E, Rb/HDAC1, Raf-1/MEK1, or Raf-1/MEK2 interaction. Suppresses cellular E2F transcription activity and reduces serum-induced Rb phosphorylation. Shown to display Rb-dependent antitumor activity both in cultures in vitro and in mice in vivo.
FormWhite solid
Intert gas (Yes/No) Packaged under inert gas
CAS number72214-67-6
Chemical formulaC₈H₉Cl₃N₂S
Structure formulaStructure formula
Purity≥97% by HPLC
SolubilityDMSO (50 mg/ml) or Ethanol (20 mg/ml)
Storage Protect from light
+2°C to +8°C
Hygroscopic
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C. However, it is strongly recommended that stock solutions be prepared fresh just prior to each use.
Toxicity Irritant
ReferencesKinkade, R., et al. 2008. Cancer Res. 68, 3810.