Qty	Catalogue Number	Ordering Description	Qty/Pack	List Price
			96-Well Plate

Qty	Catalogue Number	Ordering Description	Qty/Pack	List Price
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

QIA127 Sigma-AldrichRapid Cell Proliferation Kit

Rapid Cell Proliferation Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Recommended Products

KP20A10TT1 Millipore
Opticap^® XL10 Lifegard™ 2.0 µm 1-1/2 in. TC/TC
SVSCA47FH3 Millipore
Optiscale^® Durapore^® Multimedia 1.2/0.5/0.22 µm TC/HB
137672 SAFC
SyntheChol® Synthetic Cholesterol, For Cell Culture
219265 Sigma-Aldrich
Catalase Assay Kit
CC302 Sigma-Aldrich
PureCol® Coated 6-Well Plates
1096211000 Supelco
Ethylene glycol
1090734000 Supelco
Sulfuric acid
14-545M Sigma-Aldrich
NEK2 Protein, active, 250 µg
3549702SA Millipore
BED SUPPORT, OD 1023 X 4.75MM, 30µM, PE, CENTRE HOLE DIA 12.5MM

Detection Methods
Colorimetric

Catalogue Number	Packaging	Qty/Pack
QIA127-1KIT	Glass bottle	1 kit	Add To Favorites Add To Favorites Added To My Favorites

Description
Overview	Assay measures the increased activity of cellular mitochondrial dehydrogenases that can cleave the tetrazolium dye WST-1 to formazan. The formazan formation is then quantified by measuring the change in absorbance at 450 nm in a microplate reader. The activity of mitochondrial dehydrogenases is proportional to cell number. No washing, harvesting, or solubilization steps are required.
Catalogue Number	QIA127
Brand Family	Calbiochem®
Materials Required but Not Delivered	• Pipettors with disposable tips • Tissue culture grade, flat-bottom 96-well plate • Spectrophotometer capable of measuring absorbance in 96-well plates at a wavelength of 440-460 nm

References
References	Ishiyama, M., et al. 1995. In Vitro Toxicology 8, 187. Liu, S.O., et al 1995. Nat. Med. 1, 267. Ishiyama, M., et al. 1993. Chem. Pharm. Bull. 41, 1118.

Product Information
Detection method	Colorimetric
Form	500 Tests
Format	96-well plate
Kit contains	WST-1, 1-Methoxy PMS, and a user protocol.
Quality Level	MQ100

Physicochemical Information
Sensitivity	1000 cells (adherent); 2500 cells (nonadherent)

Product Usage Statements
Intended use	The Calbiochem^® Rapid Cell Proliferation Kit is designed to determine the number of viable cells in proliferation, or cytotoxicity studies, based on the cleavage of the tetrazolium salt WST-1 by mitochondrial dehydrogenases in viable cells. The assay can be used to measure cell proliferation in response to growth factors or cytokines, or to assess cytotoxic compounds.

Storage and Shipping Information
Ship Code	Dry Ice Only
Toxicity	Multiple Toxicity Values, refer to MSDS
Storage	-20°C
Storage Conditions	Upon arrival store the entire contents of the kit at -20°C.
Protect from Light	Protect from light
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze

Supplemental Information
Kit contains	WST-1, 1-Methoxy PMS, and a user protocol.

Global Trade Item Number
Catalogue Number	GTIN
QIA127-1KIT	07790788054021

Select A Species	.
Select A Panel Type	.
Select A Kit	.
Select A Sample Type	For some panels, sample type will determine which analytes can be plexed together.

PRODUCTS Products by Application Products by Brand Products by Industry Products by Type Ordering our Products	SUPPORT Help Feedback Cookies Customer & Tech Service FAQ Patents Contact Us	COMPANY About Us News Events Careers Change Country or Region	Research. Development. Production. We are a leading supplier to the global Life Science industry: solutions and services for research, development and production of biotechnology and pharmaceutical drug therapies.


Merck Group Imprint Terms of Use Privacy Statement Conditions of Sale

© 2025 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.Cookie Settings