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122643 ProteoExtract® Albumin/IgG Removal Kit, Maxi

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Overview

Replacement Information
Description
OverviewHigh capacity, reusable columns specifically designed for the rapid and efficient removal of >80% of albumin (column 1 containing albumin-affinity resin) and IgG (column 2 containing immobilized protein A) from body fluid samples, either on an LC-instrument or manually, attached to a syringe. Removal of these high abundance proteins allows enhanced detection of low abundance proteins.
Catalogue Number122643
Brand Family Calbiochem®
Materials Required but Not Delivered• Micropipettes and tips, 10 µl, 200 µl and 1 ml size
• Syringes with different volumes for manual usage.
• High quality water (resistance > 18 mΩ)
• Sample collection tubes e.g. 15ml centrifuge tubes
References
ReferencesBoyle, M. D. P. and K. J. Reis., 1987. Biotechnology 5, 697.
Product Information
Form20 Albumin-containing samples (60-180 μl each) and 10 IgG-containing samples (60-180 μl each)
FormatAffinity column
Kit containsTwo Albumin Removal Columns, one IgG Removal Column, 10X Albumin/IgG Binding Buffer, Albumin Elution Buffer, IgG Elution Buffer, and a user protocol.
Applications
Biological Information
Sample TypeSerum, plasma, CSF
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Intended useThe ProteoExtract® Albumin/IgG Removal Kit, Maxi provides a convenient, reproducible and highly specific method to remove serum albumin and immunoglobulins from body fluid samples. The kit provides columns containing either a novel albumin-specific affinity resin or a proprietary immobilized protein A polymeric resin. These columns allow for the removal of more than 80% of both serum albumin and immunoglobulins from human body fluid samples when used according to the instructions provided in this manual. The column hardware is designed to allow for both manual and LC-instrument controlled usage. When used manually, the columns are operated as syringe-tip filters, when used with LC-instrument control, the columns can be connected to any standard HPLC/FPLC instrument via a 10/32" port using the provided adapters. For guidelines of column connections and operation, please refer to section 3 of this manual.
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage +2°C to +8°C
Storage ConditionsUpon arrival store entire contents of the kit at 4°C. Avoid freezing of the ProteoExtract® Blue and ProteoExtract® Protein A columns! Freezing thawing will destroy the affinity resin and the columns no longer function.
Protect from Light Protect from light
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Kit containsTwo Albumin Removal Columns, one IgG Removal Column, 10X Albumin/IgG Binding Buffer, Albumin Elution Buffer, IgG Elution Buffer, and a user protocol.
Specifications
Global Trade Item Number
Catalogue Number GTIN
122643 0

Documentation

References

Reference overview
Boyle, M. D. P. and K. J. Reis., 1987. Biotechnology 5, 697.

User Protocols

Title
User Protocol