Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

325540 Protein Tyrosine Kinase Positive Control, Rat Spleen Extract

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
325540
  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Overview

This product has been discontinued.





Has been validated for use as a positive control for protein tyrosine kinase (PTK) activity assays. There is sufficient PTK activity at a concentration of 10-20 ng/well using the K-LISA™ PTK Screening Kit (Cat. No. 539701).
Catalogue Number325540
Brand Family Calbiochem®
Application Data
The rat spleen extract was reconstituted as indicated above, diluted in reaction buffer, and assayed using the K-LISA™ PTK Screening Kit according to the recommended protocol. Absorbance values shown represent the mean of triplicate samples that were corrected against background absorbance. Background absorbance values ranged from ~0.05-0.07 absorbance units.
References
ReferencesElberg, G. et al., 1995. Biochim. Biophys. Acta. 1269, 299.
Swarup, G. et al. 1983. J. Biol. Chem. 258, 10341.
Product Information
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
325540 0

Documentation

References

Reference overview
Elberg, G. et al., 1995. Biochim. Biophys. Acta. 1269, 299.
Swarup, G. et al. 1983. J. Biol. Chem. 258, 10341.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision20-September-2008 JSW
Application Data
The rat spleen extract was reconstituted as indicated above, diluted in reaction buffer, and assayed using the K-LISA™ PTK Screening Kit according to the recommended protocol. Absorbance values shown represent the mean of triplicate samples that were corrected against background absorbance. Background absorbance values ranged from ~0.05-0.07 absorbance units.
DescriptionThe Protein Tyrosine Kinase Positive Control, Rat Spleen Extract, 7 mg has been validated for use as a positive control for protein tyrosine kinase (PTK) activity assays. There is sufficient PTK activity at a concentration of 10-20 ng/well using the K-LISA™ PTK Screening Kit (Cat. No. 539701).

Assay Characteristics and Examples

The assay example below represents typical data obtained using the rat spleen extract in a tyrosine kinase assay. Actual tyrosine kinase activity may vary from lot to lot.
Recommended reaction conditionsReagent Preparation Reconstitute the entire vial of rat spleen extract with 250 µl of ultra pure H2O. The resulting suspension has a concentration of 28 mg/ml total protein. Dispense into aliquots and store at -70°C. Use one aliquot as positive control for tyrosine kinase assays. There is sufficient PTK activity at 10-20 ng/well to serve as a strong positive control (see example below). Discard the unused portion; do not re-freeze.
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Toxicity Standard Handling
ReferencesElberg, G. et al., 1995. Biochim. Biophys. Acta. 1269, 299.
Swarup, G. et al. 1983. J. Biol. Chem. 258, 10341.