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539486 Protein Kinase A, Catalytic Subunit, Bovine Heart

Convenient kit for site-specific ³²P-labeling of target proteins

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: cAMP-Dependent Protein Kinase Catalytic Subunit

539486
  
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Overview

Replacement Information
Description
OverviewThe PKAce™ Kit is designed for in vitro labeling of proteins in a simple reaction with 32P-γ-ATP and purified Protein Kinase A (PKA). Proteins can be labeled to at least 105 cpm/µg in 10 minutes, and can be used directly as sensitive and specific probes for protein:protein interactions (1). The PKAce Kit includes a highly purified preparation of bovine heart PKA, optimized reaction buffer, and PKA Control Protein for verification of kit performance.
Mediates the majority of the actions of cAMP. Regulates a number of cellular functions including gene expression, carbohydrate metabolism, Ca2+ homeostasis, and ion channel function. Stimulates Ca2+-dependent exocytosis in bovine chromaffin cells. Suitable for studies of PKA phosphorylation in vitro. Unlike the holoenzyme, the catalytic subunit does not require cAMP for activation.
Catalogue Number539486
Brand Family Calbiochem®
SynonymscAMP-Dependent Protein Kinase Catalytic Subunit
References
ReferencesRazani, B., et al. 1999. J. Biol. Chem. 274, 26353. Gross, R.A., et al. 1994. Mol. Endocrinol. 8, 970. Bosma, M.M., et al. 1993. J. Neurosci. 13, 5242. Brindle, P., et al. 1993. Nature 364, 821. Hajnoczky, G., et al. 1993. Biochem. J. 293, 413. Sculptoreanu, A., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 10135. Wang, L-Y., et al. 1993. Science 259, 1173.
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme that will transfer 1.0 pmol phosphate to histone per min at 30°C.
EC number2.7.1.37
FormLiquid
FormulationIn 100 mM NaCl, 30 mM DTT, 20 mM MES, 100 µM EDTA, 50% glycerol, pH 6.5.
Applications
Biological Information
Purity≥95% by SDS-PAGE
Specific Activity≥750 units/µg protein
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage DEEP FREEZE (-70°C)
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
539486 0

Documentation

Protein Kinase A, Catalytic Subunit, Bovine Heart Certificates of Analysis

TitleLot Number
539486

References

Reference overview
Razani, B., et al. 1999. J. Biol. Chem. 274, 26353. Gross, R.A., et al. 1994. Mol. Endocrinol. 8, 970. Bosma, M.M., et al. 1993. J. Neurosci. 13, 5242. Brindle, P., et al. 1993. Nature 364, 821. Hajnoczky, G., et al. 1993. Biochem. J. 293, 413. Sculptoreanu, A., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 10135. Wang, L-Y., et al. 1993. Science 259, 1173.