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PAT001-100UG Protein A-Turbo - Proximity Biotinylation Enzyme

Proximity Biotinylation - Protein A Fusion Enzyme, Cat. No. PAT001. Proximity biotinylation is a tool for probing protein-protein interactions. It is used in Affinity Binding Assay, Immunocytochemistry, Proximity Ligation Assay, IP, and Mass Spec.

PAT001-100UG
100 μg  Liquid
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberPAT001-100UG
DescriptionProtein A-Turbo - Proximity Biotinylation Enzyme
Alternate Names
  • ProteinA-Turbo ID
Background InformationProtein A Turbo is a recombinantly expressed Protein A-TurboID fusion protein with the capability to bind IgG through the Protein A region, and enzymatically biotinylate proximal proteins non-specifically with the TurboID region.
References
Product Information
FormatPurified
PresentationPurified protein in 20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM Spermidine, 150 mM imidazole with protease inhibitor cocktail.
Quality LevelMQ200
Applications
ApplicationProximity Biotinylation - Protein A Fusion Enzyme, Cat. No. PAT001. Proximity biotinylation is a tool for probing protein-protein interactions. It is used in Affinity Binding Assay, Immunocytochemistry, Proximity Ligation Assay, IP, and Mass Spec.
Key Applications
  • Immunofluorescence
  • Chromatography
  • Mass Spectrometry
  • Immunoprecipitation
Application Notes- Proximity protein labeling
- Cell Biology
- Histology
- Fixed cell microscopy
- Tissue staining
- Immunofluorescence

-Liquid Chromatography

- Mass Spectrometry

-Immunoprecipitation
Biological Information
Concentration0.7 mg/mL
SpecificityProtein A strongly binds multiple Immunoglobulin G subclasses (IgG1, IgG2, IgG4), but does not bind IgG3. This probe is not compatible with primary antibodies that are IgG3.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEnzyme was tested for proximity biotinylation in cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -65°C to -96°C.
Packaging Information
Material Size100 μg
Material PackageLiquid
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
PAT001-100UG 04065270500014

Documentation

Protein A-Turbo - Proximity Biotinylation Enzyme SDS

Title

Safety Data Sheet (SDS) 

Protein A-Turbo - Proximity Biotinylation Enzyme Certificates of Analysis

TitleLot Number
Protein A-Turbo - Proximity Biotinylation Enzyme - Q4083389 Q4083389
Protein A-Turbo - Proximity Biotinylation Enzyme - Q4083395 Q4083395

References

Reference overviewPub Med ID
Off-the-shelf proximity biotinylation for interaction proteomics
Santos-Barriopedro I, et al.
Nature Communications  12  1270-1281  2021

34408139 34408139