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524737 Phosphodiesterase 4C2, His•Tag®, Human, Recombinant, S. frugiperda

524737
  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Overview

This product has been discontinued.





Full-length, recombinant, human phosphodiesterdase 4C2 (accession no. U88712) fused at the N-terminus to a His•Tag® sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. Supplied as a partially purified preparation of the catalytically active enzyme.
Catalogue Number524737
Brand Family Calbiochem®
SynonymsPDE4C2, cAMP-Specific Phosphodiesterase 4C2
Application Data
Phosphodiesterase 4C2 was treated with increasing concentration of the inhibitor, rolipram, and the activity assayed. the IC50 for this inhibition is 7.87 µM.
References
ReferencesGibson, L.C.D., et al. 2006. Eur. J. Pharmacol. 538, 39.
Sullivan, M., et al. 1999. Cell Signal. 11, 735.
Engels, P., et al. 1995. FEBS Lett. 358, 305.
Product Information
Activity≥ 0.5 units/µl
Unit of DefinitionOne unit is defined as the amount of enzyme that will convert 1 pmol cAMP to AMP per min at 30°C, pH 7.5.
FormLiquid
FormulationIn 150 mM NaCl, 10 mM Tris-HCl, 0.1% NP-40, pH 8.3.
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
524737 0

Documentation

Phosphodiesterase 4C2, His•Tag®, Human, Recombinant, S. frugiperda SDS

Title

Safety Data Sheet (SDS) 

Phosphodiesterase 4C2, His•Tag®, Human, Recombinant, S. frugiperda Certificates of Analysis

TitleLot Number
524737

References

Reference overview
Gibson, L.C.D., et al. 2006. Eur. J. Pharmacol. 538, 39.
Sullivan, M., et al. 1999. Cell Signal. 11, 735.
Engels, P., et al. 1995. FEBS Lett. 358, 305.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision06-March-2009 JSW
SynonymsPDE4C2, cAMP-Specific Phosphodiesterase 4C2
Application Data
Phosphodiesterase 4C2 was treated with increasing concentration of the inhibitor, rolipram, and the activity assayed. the IC50 for this inhibition is 7.87 µM.
DescriptionFull-length, recombinant, human phosphodiesterdase 4C2 (accession no. U88712) fused at the N-terminus to a His•Tag® sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. Supplied as a partially purified preparation of the catalytically active enzyme.
FormLiquid
FormulationIn 150 mM NaCl, 10 mM Tris-HCl, 0.1% NP-40, pH 8.3.
Activity≥ 0.5 units/µl
Unit definitionOne unit is defined as the amount of enzyme that will convert 1 pmol cAMP to AMP per min at 30°C, pH 7.5.
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesGibson, L.C.D., et al. 2006. Eur. J. Pharmacol. 538, 39.
Sullivan, M., et al. 1999. Cell Signal. 11, 735.
Engels, P., et al. 1995. FEBS Lett. 358, 305.