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PS1024 PhosphoDetect™ Anti-Smad1/5 (pSer463/465) Rabbit mAb (41D10)

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PS1024
  
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Overview

Replacement Information

Key Spec Table

Host
Rb
Description
Overview

This product has been discontinued.





Recognizes the ~60 kDa Smad1/5 protein phosphorylated at Ser463/465 in HeLa cells treated with BMP-4.
Catalogue NumberPS1024
Brand Family Calbiochem®
Application Data
Detection of human Smad1/5, phosphorylated at pSer463/465, by immunoblotting. Samples: whole cell lysates from HeLa or NIH/3T3 cells, left untreated or treated with BMP-4, as indicated by the (+) and (-). Primary antibody: PhosphoDetect™ Anti-Smad1/5 (pSer463/465) Rabbit mAb (41D10) (Cat. No. PS1024) (1:1000). Detection: chemiluminescence.

Detection of human Smad1/5 phosphorylated at pSer463/465, by flow cytomtry. Samples: HeLa cells left untreated (blue) or treated with BMP-4 (green). Primary antibody: PhosphoDetect™ Anti-Smad1/5 (pSer463/465) Rabbit mAb (41D10) (Cat. No. PS1024) (1:200) (blue and green) and a nonspecific, negative control antibody (red). Detection: fluorescence.
References
ReferencesKlemm, J.D., et al. 1998. Annu. Rev. Immunol. 16, 569.
Kretzschumar, M., et al. 1997. Genes Dev. 11, 984.
Hoodless, P.A., et al. 1996. Cell 296, 1646.
Product Information
FormLiquid
FormulationIn 50% glycerol, 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, pH 7.5.
Preservative≤0.1 sodium azide
Applications
Key Applications Flow Cytometry
Immunoblotting (Western Blotting)
Application NotesImmunoblotting (1:1000)
Flow Cytometry (1:200)
Application CommentsDoes not cross-react with other Smad proteins. For immunoblotting incubate membrane with antibody diluted with TBS containing 5% BSA, 0.1% Tween®-20 detergent overnight at 4°C with gentle shaking. Please note that this is a rabbit monoclonal antibody and requires the use of a secondary antibody. Antibody should be titrated for optimal results in individual sytems.
Biological Information
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser 463/465 phosphorylation sites of human Smad5, coupled to KLH.
ImmunogenHuman
Clone41D10
HostRabbit
IsotypeIgG
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsDo not aliquot.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
PS1024 0

Documentation

PhosphoDetect™ Anti-Smad1/5 (pSer463/465) Rabbit mAb (41D10) Certificates of Analysis

TitleLot Number
PS1024

References

Reference overview
Klemm, J.D., et al. 1998. Annu. Rev. Immunol. 16, 569.
Kretzschumar, M., et al. 1997. Genes Dev. 11, 984.
Hoodless, P.A., et al. 1996. Cell 296, 1646.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision14-May-2009 JSW
ApplicationImmunoblotting (1:1000)
Flow Cytometry (1:200)
Application Data
Detection of human Smad1/5, phosphorylated at pSer463/465, by immunoblotting. Samples: whole cell lysates from HeLa or NIH/3T3 cells, left untreated or treated with BMP-4, as indicated by the (+) and (-). Primary antibody: PhosphoDetect™ Anti-Smad1/5 (pSer463/465) Rabbit mAb (41D10) (Cat. No. PS1024) (1:1000). Detection: chemiluminescence.

Detection of human Smad1/5 phosphorylated at pSer463/465, by flow cytomtry. Samples: HeLa cells left untreated (blue) or treated with BMP-4 (green). Primary antibody: PhosphoDetect™ Anti-Smad1/5 (pSer463/465) Rabbit mAb (41D10) (Cat. No. PS1024) (1:200) (blue and green) and a nonspecific, negative control antibody (red). Detection: fluorescence.
DescriptionPurified rabbit monoclonal antibody generated by immunizing rabbits with the specified immunogen and fusing splenocytes with an immortal cell line. Recognizes the ~60 kDa Smad1/5 protein phosphorylated at Ser463/465.
BackgroundBone morphogenetic proteins (BMPs) constitute a large family of signaling molecules that regulate a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation, and apoptosis. BMP receptors are members of the TGF-β family of Ser/Thr kinase receptors. Ligand binding induces multimerization, autophosphorylation, and activation of these receptors. Subsequently, the activated receptor phosphorylate Smad1 at Ser463 and Ser465 in the C-terminal motif, SSXS, as well as Smad5 and Smad8 at their corresponding sites. These phosphorylated Smads dimerize with Smad4 and translocate to the nucleus, where the transcription of target genes is stimulated.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser 463/465 phosphorylation sites of human Smad5, coupled to KLH.
Clone41D10
IsotypeIgG
Specieshuman, mouse
FormLiquid
FormulationIn 50% glycerol, 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, pH 7.5.
Preservative≤0.1 sodium azide
CommentsDoes not cross-react with other Smad proteins. For immunoblotting incubate membrane with antibody diluted with TBS containing 5% BSA, 0.1% Tween®-20 detergent overnight at 4°C with gentle shaking. Please note that this is a rabbit monoclonal antibody and requires the use of a secondary antibody. Antibody should be titrated for optimal results in individual sytems.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsDo not aliquot.
Toxicity Standard Handling
ReferencesKlemm, J.D., et al. 1998. Annu. Rev. Immunol. 16, 569.
Kretzschumar, M., et al. 1997. Genes Dev. 11, 984.
Hoodless, P.A., et al. 1996. Cell 296, 1646.