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PS1014 PhosphoDetect™ Anti-JAK2 (pTyr1007/1008) Rabbit pAb

This PhosphoDetect™ Anti-JAK2 (pTyr1007/1008) Rabbit pAb is validated for use in Immunoblotting for the detection of JAK2 (pTyr1007/1008).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Janus Kinase 2, Phospho-Specific (Tyr1007/1008

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PS1014
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RRbPolyclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the ~130 kDa JAK2 protein phosphorylated at Tyr1007/1008 in LIF-treated 3T3-L1 cells.
Catalogue NumberPS1014
Brand Family Calbiochem®
SynonymsAnti-Janus Kinase 2, Phospho-Specific (Tyr1007/1008
Application Data
Detection of mouse JAK2, phosphorylated at Tyr1007/1008, by immunoblotting. Samples: Whole cell lysate from NIH3T3-L1 cells left unstimulated (lane 1) or stimulated with leukemia inhibitor factor (LIF) (lanes 2-4). Primary antibody: PhosphoDetect™ Anti-JAK2 (Tyr1007/1008) Rabbit pAb (Cat. No. PS1014) (1:1000) pre-incubated without peptide (lanes 1,2), with non-phosphopeptide corresponding to the immunogen (lane 3), or the phosphopeptide immunogen (lane 4). Detection: chemiluminescence.
References
ReferencesFrank, G.D., et al. 2003. Mol. Biol. 23, 1581.
Kaszubska, W., et al. 2002. Mol. Cell. Endocrinol. 195, 109.
Kirito, K., et al. 2002. J. Biol. Chem. 277, 8329.
Shaw, S., et al. 2002. J. Biol. Chem. 277, 44920.
Sutherland, C.L., et al. 2002. J. Immunol. 168, 671.
Zeng, Z.Z., et al. 2002. J. Biol. Chem. 277, 41213.
Eguchi, S., et al. 2001. J. Biol. Chem. 276, 7957.
Madamanchi, N.R., et al. 2001. J. Biol. Chem. 276, 18915.
Myers, M.P., et al. 2001. J. Biol. Chem. 276, 47771.
Madamanchi, N.R., et al. 2000. Arterioscler. Thromb. Vasc. Biol. 21, 321.
Amiri, F., et al. 1999. J. Biol. Chem. 274, 32382.
Ihle, J.N., et al. 1996. Cell 84, 331.
Product Information
FormLiquid
FormulationIn Dulbecco's PBS (without Mg2+ and Ca2+), 50 % glycerol, 1 mg/ml BSA, pH 7.3 (± 0.1).
Positive control3T3-L1 adipocytes stimulated with leukemia inhibitor factor (LIF)
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (1:1000)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr1007/1008 phosphorylation sites of human JAK2
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Irritant
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
PS1014 0

Documentation

PhosphoDetect™ Anti-JAK2 (pTyr1007/1008) Rabbit pAb SDS

Title

Safety Data Sheet (SDS) 

PhosphoDetect™ Anti-JAK2 (pTyr1007/1008) Rabbit pAb Certificates of Analysis

TitleLot Number
PS1014

References

Reference overview
Frank, G.D., et al. 2003. Mol. Biol. 23, 1581.
Kaszubska, W., et al. 2002. Mol. Cell. Endocrinol. 195, 109.
Kirito, K., et al. 2002. J. Biol. Chem. 277, 8329.
Shaw, S., et al. 2002. J. Biol. Chem. 277, 44920.
Sutherland, C.L., et al. 2002. J. Immunol. 168, 671.
Zeng, Z.Z., et al. 2002. J. Biol. Chem. 277, 41213.
Eguchi, S., et al. 2001. J. Biol. Chem. 276, 7957.
Madamanchi, N.R., et al. 2001. J. Biol. Chem. 276, 18915.
Myers, M.P., et al. 2001. J. Biol. Chem. 276, 47771.
Madamanchi, N.R., et al. 2000. Arterioscler. Thromb. Vasc. Biol. 21, 321.
Amiri, F., et al. 1999. J. Biol. Chem. 274, 32382.
Ihle, J.N., et al. 1996. Cell 84, 331.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision17-October-2007 RFH
SynonymsAnti-Janus Kinase 2, Phospho-Specific (Tyr1007/1008
ApplicationImmunoblotting (1:1000)
Application Data
Detection of mouse JAK2, phosphorylated at Tyr1007/1008, by immunoblotting. Samples: Whole cell lysate from NIH3T3-L1 cells left unstimulated (lane 1) or stimulated with leukemia inhibitor factor (LIF) (lanes 2-4). Primary antibody: PhosphoDetect™ Anti-JAK2 (Tyr1007/1008) Rabbit pAb (Cat. No. PS1014) (1:1000) pre-incubated without peptide (lanes 1,2), with non-phosphopeptide corresponding to the immunogen (lane 3), or the phosphopeptide immunogen (lane 4). Detection: chemiluminescence.
DescriptionRabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~130 kDa JAK2 protein.
BackgroundJanus Kinase 2 (JAK2) belongs the the JAK family of non-receptor tyrosine kinases. The JAK family consists of four members, JAK1, JAK2, JAK3, and TYK2. Ligand binding to a variety of cell surface receptors, like cytokine and growth factor receptors, results in the association and subsequent activtion of JAK kinases via phosphorylation. Activated JAK proteins, in turn, phosphorylate members of the Signal Transducers and Activators of Transcription (STAT) family of transcription factors. In the JAK2 domain, there is a region that has considerable sequence homology to the regulatory region of the insulin receptor and contains two tyrosines, Tyr1007 and Tyr1008, that are key phoshorylation/regulatory sites.
HostRabbit
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr1007/1008 phosphorylation sites of human JAK2
IsotypeIgG
Specieshuman, mouse, rat
Positive control3T3-L1 adipocytes stimulated with leukemia inhibitor factor (LIF)
FormLiquid
FormulationIn Dulbecco's PBS (without Mg2+ and Ca2+), 50 % glycerol, 1 mg/ml BSA, pH 7.3 (± 0.1).
Preservative≤0.1% sodium azide
CommentsAntibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Irritant
ReferencesFrank, G.D., et al. 2003. Mol. Biol. 23, 1581.
Kaszubska, W., et al. 2002. Mol. Cell. Endocrinol. 195, 109.
Kirito, K., et al. 2002. J. Biol. Chem. 277, 8329.
Shaw, S., et al. 2002. J. Biol. Chem. 277, 44920.
Sutherland, C.L., et al. 2002. J. Immunol. 168, 671.
Zeng, Z.Z., et al. 2002. J. Biol. Chem. 277, 41213.
Eguchi, S., et al. 2001. J. Biol. Chem. 276, 7957.
Madamanchi, N.R., et al. 2001. J. Biol. Chem. 276, 18915.
Myers, M.P., et al. 2001. J. Biol. Chem. 276, 47771.
Madamanchi, N.R., et al. 2000. Arterioscler. Thromb. Vasc. Biol. 21, 321.
Amiri, F., et al. 1999. J. Biol. Chem. 274, 32382.
Ihle, J.N., et al. 1996. Cell 84, 331.