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382156 PhosphoDetect™ Anti-Histone H1 Rabbit pAb

PhosphoDetect™ Anti-Histone H1 Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
382156
  
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Overview

Key Spec Table

Host
Rb
Description
Overview

This product has been discontinued.





Recognizes the ~32 kDa phosphorylated histone H1 protein. Also detects proteins at ~64 kDa and ~130 kDa.
Catalogue Number382156
Brand Family Calbiochem®
References
ReferencesPennisi, E. 1997. Science 275, 155.
Lu, M.J., et al. 1994. Chromosoma 103, 111.
Dedon, P.C., et al. 1991. Anal. Biol. Chem. 197, 83.
Product Information
FormLiquid
Formulation105 mM NaCl, 70 mM Tris-glycine, 30% glycerol, pH 7.4.
Preservative≤0.1% sodium azide
Applications
Key Applications Immunoblotting (Western Blotting)
Immunocytochemistry
Not Immunoprecipitation
Application NotesImmunoblotting (1 µg/ml)
Immunocytochemistry (5-10 µg/ml)
Immunoprecipitation (not recommended)
Application CommentsIt has not yet been determined which specific histone H1 phosphorylation sites this antibody detects. Does not cross-react with unphosphorylated histone H1. Also detects bands at ~64 kDa and ~130 kDa that have been shown to contain histone H1 using anti-histone H1 blotting. For immunocytochemistry, colcemid-treated HeLa cells were fixed with 2% paraformaldehyde followed by cold methanol. Variables associated with assay conditions will dictate the optimal working dilution.
Biological Information
Immunogena hyperphosphorylated isoform of Tetrahymena histone H1
ImmunogenTetrahymena
HostRabbit
IsotypeIgG
Concentration Label Please refer to vial label for lot-specific concentration
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Global Trade Item Number
Catalogue Number GTIN
382156 0