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382156 PhosphoDetect™ Anti-Histone H1 Rabbit pAb

PhosphoDetect™ Anti-Histone H1 Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
382156
  
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Overview

Replacement Information

Key Spec Table

Host
Rb
Description
Overview

This product has been discontinued.





Recognizes the ~32 kDa phosphorylated histone H1 protein. Also detects proteins at ~64 kDa and ~130 kDa.
Catalogue Number382156
Brand Family Calbiochem®
References
ReferencesPennisi, E. 1997. Science 275, 155.
Lu, M.J., et al. 1994. Chromosoma 103, 111.
Dedon, P.C., et al. 1991. Anal. Biol. Chem. 197, 83.
Product Information
FormLiquid
Formulation105 mM NaCl, 70 mM Tris-glycine, 30% glycerol, pH 7.4.
Preservative≤0.1% sodium azide
Applications
Key Applications Immunoblotting (Western Blotting)
Immunocytochemistry
Not Immunoprecipitation
Application NotesImmunoblotting (1 µg/ml)
Immunocytochemistry (5-10 µg/ml)
Immunoprecipitation (not recommended)
Application CommentsIt has not yet been determined which specific histone H1 phosphorylation sites this antibody detects. Does not cross-react with unphosphorylated histone H1. Also detects bands at ~64 kDa and ~130 kDa that have been shown to contain histone H1 using anti-histone H1 blotting. For immunocytochemistry, colcemid-treated HeLa cells were fixed with 2% paraformaldehyde followed by cold methanol. Variables associated with assay conditions will dictate the optimal working dilution.
Biological Information
Immunogena hyperphosphorylated isoform of Tetrahymena histone H1
ImmunogenTetrahymena
HostRabbit
IsotypeIgG
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
382156 0

Documentation

PhosphoDetect™ Anti-Histone H1 Rabbit pAb Certificates of Analysis

TitleLot Number
382156

References

Reference overview
Pennisi, E. 1997. Science 275, 155.
Lu, M.J., et al. 1994. Chromosoma 103, 111.
Dedon, P.C., et al. 1991. Anal. Biol. Chem. 197, 83.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision26-February-2008 JSW
ApplicationImmunoblotting (1 µg/ml)
Immunocytochemistry (5-10 µg/ml)
Immunoprecipitation (not recommended)
DescriptionProtein A purified rabbit polyclonal antibody. Recognizes the ~32 kDa phosphorylated form of histone H1 protein.
BackgroundHistone H1, the nonnucleosome linker histone, becomes phosphorylated at multiple sites (3-6 sites/molecule of H1) upon entry of the cell into mitosis, most likely by Cdc2 kinase. It has been proposed that H1 phosphorylation may allow for transient chromatin decondensations, thereby allowing access of specific DNA binding factors involved in gene activation, DNA replication and chromosome condensation to the DNA.
HostRabbit
Immunogen speciesTetrahymena
Immunogena hyperphosphorylated isoform of Tetrahymena histone H1
IsotypeIgG
Speciesbovine, human
FormLiquid
Formulation105 mM NaCl, 70 mM Tris-glycine, 30% glycerol, pH 7.4.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsIt has not yet been determined which specific histone H1 phosphorylation sites this antibody detects. Does not cross-react with unphosphorylated histone H1. Also detects bands at ~64 kDa and ~130 kDa that have been shown to contain histone H1 using anti-histone H1 blotting. For immunocytochemistry, colcemid-treated HeLa cells were fixed with 2% paraformaldehyde followed by cold methanol. Variables associated with assay conditions will dictate the optimal working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesPennisi, E. 1997. Science 275, 155.
Lu, M.J., et al. 1994. Chromosoma 103, 111.
Dedon, P.C., et al. 1991. Anal. Biol. Chem. 197, 83.