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539736 PTP1B Assay Kit, Colorimetric

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539736
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Overview

Key Spec Table

Detection Methods
Colorimetric

Products

Catalogue NumberPackaging Qty/Pack
539736-1KIT Glass bottle 1 kit
Description
OverviewA colorimetric assay kit to measure PTP1B activity. This convenient 96-well assay is useful for screening inhibitors and modulators of PTP1B activity.
Catalogue Number539736
Brand Family Calbiochem®
SynonymsProtein Tyrosine Phosphatase 1B Assay Kit, Colorimetric
Materials Required but Not Delivered Plate reader capable of measuring A620 to >3-decimal accuracy
Pipetman capable of pipetting 2-100 µl accurately
Multi-channel pipetman capable of pipetting 25 and 50 µl (optional)
Microcentrifuge
Ice bucket to keep reagents cold until use
Temperature controlled microplate warmer and/or water bath for performing assays at other than ambient temperature (optional)
References
ReferencesElchebly, M., et al. 1999. Science 283, 1544.
Puius, Y.A., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 13420.
Liu, F., et al. 1996. J. Biol. Chem. 271, 31290.
Fujii, S., et al. 1995. Neurosci. Lett. 187, 133.
Harder, K.W., et al. 1994. Biochem. J. 298, 395.
Zhang, Z. Y., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 4446.
Martin, B., et al. 1985. J. Biol. Chem. 260, 14932.
Product Information
Detection methodColorimetric
Form96 Tests
Format96-well plate
Kit containsHuman Recombinant PTP1B (Cat. No. 539735), PTP1B Substrate (Cat. No. 539737), Assay Buffer, Phosphate Detection Agent, Phosphate Standard, RK-682 Inhibitor (Cat. No. 557322), ½ Volume Plate, and a user protocol.
Positive controlRecombinant PTP1B
Quality LevelMQ100
Biological Information
Assay time2 h
Sample TypePurified enzyme
Safety Information
R PhraseR: 36/38-21/22

Irritating to eyes and skin.
Harmful in contact with skin and if swallowed.
S PhraseS: 26-24/25

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Avoid contact with skin and eyes.
Product Usage Statements
Intended usePLEASE READ THE ENTIRE PROTOCOL BEFORE PROCEEDING WITH THE ASSAY. CAREFULLY NOTE THE HANDLING AND STORAGE CONDITIONS OF EACH KIT COMPONENT. PLEASE CONTACT CALBIOCHEM® TECHNICAL SERVICES FOR ASSISTANCE IF NECESSARY.

NOTE: THE FOLLOWING PROCEDURES ARE INTENDED ONLY AS A GUIDELINE. THE OPTIMAL EXPERIMENTAL CONDITIONS WILL VARY DEPENDING ON THE PARAMETERS BEING INVESTIGATED, AND MUST BE DETERMINED BY THE INDIVIDUAL USER.


The Calbiochem® PTP1B Assay Kit, Colorimetric is a nonradioactive assay designed for measuring PTP1B activity in purified prperations and for inhibitor or activator screening.
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage ≤ -70°C
Storage ConditionsUpon arrival store the 1/2 Volume Plate at room temperature and the remaining components at -70°C to maintain the highest stability. The PTP1B must be handled very carefully in order to retain maximal enzymatic activity. Thaw it quickly in a RT water bath or by rubbing between fingers, then immediately store on an ice bath. The remaining unused enzyme should be quickly refrozen by placing at -70°C. To minimize the number of freeze/thaw cycles, aliquot the PTP1B into separate tubes and store at -70°C.
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Supplemental Information
Kit containsHuman Recombinant PTP1B (Cat. No. 539735), PTP1B Substrate (Cat. No. 539737), Assay Buffer, Phosphate Detection Agent, Phosphate Standard, RK-682 Inhibitor (Cat. No. 557322), ½ Volume Plate, and a user protocol.
Global Trade Item Number
Catalogue Number GTIN
539736-1KIT 04055977194296