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14-782 PI 3-Kinase (p110α(E542K)/p85α), Human, 10 µg

Complex of N-terminal 6His-tagged, recombinant, full-length human p110α containing the mutation E542K & untagged, recombinant, full-length human p85α. For use in Kinase Assays.

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14-782
10 µg  Also available in 250 μg size --call for pricing and availability and reference catalog number 14-782M when ordering the 250 μg size.
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Overview

Description
Catalogue Number14-782
Trade Name
  • Upstate
DescriptionPI 3-Kinase (p110α(E542K)/p85α), Human, 10 µg
OverviewComplex of N-terminal 6His-tagged, recombinant, full-length human p110α containing the mutation E542K and untagged, recombinant, full-length human p85α.
Background InformationThe E542K substitution is a somatic mutation in p110α that has been associated with tumours of the colon and brain. Combined in vitro and in vivo studies have shown that this mutation confers higher lipid kinase activity than wild type, and is able to induce oncogenic transformation.
Product Information
Quality LevelMQ100
Applications
ApplicationComplex of N-terminal 6His-tagged, recombinant, full-length human p110α containing the mutation E542K & untagged, recombinant, full-length human p85α. For use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceCoexpressed by baculovirus in Sf21 insect cells.
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryPhosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers.
Gene Symbol
  • PIK3CA
  • PI3K
  • MGC142161
  • p110-alpha
  • MGC142163
Protein TargetPI3Kα/p85α
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyPI3K/PI4K
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P42336 # Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2.
SIZE: 1068 amino acids; 124284 Da
SUBUNIT: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunit. Binds to IRS1 in nuclear extracts. Interacts with RUFY3 (By similarity).
DISEASE: SwissProt: P42336 # Defects in PIK3CA are associated with colorectal cancer (CRC) [MIM:114500]. & Defects in PIK3CA are associated with breast cancer [MIM:114480]. & Defects in PIK3CA are associated with epithelial ovarian cancer (EOC) [MIM:604370].
SIMILARITY: SwissProt: P42336 ## Belongs to the PI3/PI4-kinase family. & Contains 1 C2 domain. & Contains 1 PI3K/PI4K domain.
Molecular Weightp110α (E542K) MW = 125.3kDa, p85α MW = 83.7kDa.
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.
Packaging Information
Material Size10 µg
Material PackageAlso available in 250 μg size --call for pricing and availability and reference catalog number 14-782M when ordering the 250 μg size.
Global Trade Item Number
Catalogue Number GTIN
14-782 04053252477126