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324881 PERK, GST-Fusion, Human, Recombinant, E. coli

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: EIF2AK3 PKR

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Overview

Description
Overview

This product has been discontinued.



Recombinant, human PERK consisting of amino acids 563-1115 fused at the N-terminus to GST and expressed in E. coli. PERK phosphorylates the α-subunit of eukaryotic translation initiation factor 2 (EIF2α), which leads to its inactivation and reduces translation initiation. The active kinase is useful for the study of PERK regulation and for inhibitor screening.
Note: the calculated MW is 88,000, but the protein runs at ~115,000 on SDS-PAGE.
Catalogue Number324881
Brand Family Calbiochem®
SynonymsEIF2AK3 PKR
Application Data
The activity of PERK was measured as outlined in the Recommended Reaction Conditions.
References
ReferencesBaltzis, D., et al. 2007. J Biol Chem. 43, 31675.
Koumenis, C., et al. 2002. Mol Cell Biol. 21, 7405.
Product Information
Unit of DefinitionKinase activity is measured as the molar amount of phosphate incorporated into SMAD3 at 30°C using a final concentration of 50 µM ATP.
FormLiquid
FormulationIn 50 mM Tris-HCl, 50 mM NaCl, 250 µM DTT, 100 µM EGTA, 100 µM EDTA, 100 µM PMSF, 25% glycerol, pH 7.5.
Quality LevelMQ100
Biological Information
Purity≥90% by SDS-PAGE
Specific ActivityPlease refer to the lot-specific Certificate of Analysis
Concentration Label Please refer to vial label for lot-specific concentration
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Global Trade Item Number
Catalogue Number GTIN
324881 0