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512777 PBSF/SDF-1β, Human, Recombinant, E. coli

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: rhPBSF/SDF-1β

512777
  
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Overview

Replacement Information
Description
Overview

This product has been discontinued.





Recombinant, human PBSF/SDF-1β expressed in E. coli. Supports the proliferation of a stromal cell-dependent pre-B cell line. Found to be a chemoattractant for T lymphocytes and monocytes but not neutrophils. Recently shown to be a ligand for the CXCR4 (fusin/LESTR) receptor that functions as a coreceptor for lymphocyte-tropic HIV-1 strains.
Catalogue Number512777
Brand Family Calbiochem®
SynonymsrhPBSF/SDF-1β
References
ReferencesBleul, C., et al. 1996. Nature 382, 829.
Oberlin, E., et al. 1996. Nature 382, 833.
Nagasawa, T., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 2305.
Tashiro, K., et al. 1993. Science 261, 600.
Product Information
FormLyophilized
FormulationLyophilized from sterile-filtered 30% acetonitrile, 0.1% TFA, 50 µg BSA/µg PBSF/SDF-1β.
Applications
Biological Information
Biological activityED₅₀ = 50-100 ng/ml as measured by its ability to chemoattract human T lymphocytes cultured in the presence of IL-2
Purity≥97% by SDS-PAGE
Physicochemical Information
ContaminantsEndotoxin: ≤100 pg/µg PBSF/SDF-1β
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long-term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
512777 0

Documentation

PBSF/SDF-1β, Human, Recombinant, E. coli Certificates of Analysis

TitleLot Number
512777

References

Reference overview
Bleul, C., et al. 1996. Nature 382, 829.
Oberlin, E., et al. 1996. Nature 382, 833.
Nagasawa, T., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 2305.
Tashiro, K., et al. 1993. Science 261, 600.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision18-August-2008 RFH
SynonymsrhPBSF/SDF-1β
DescriptionRecombinant, human PBSF/SDF-1β expressed in E. coli. Supports the proliferation of a stromal cell-dependent pre-B cell line. Found to be a chemoattractant for T-lymphocytes and monocytes but not neutrophils. Recently shown to be a ligand for the CXCR4 (fusin/LESTR) receptor that functions as a coreceptor for lymphocyte tropic HIV-1 strains.
FormLyophilized
FormulationLyophilized from sterile-filtered 30% acetonitrile, 0.1% TFA, 50 µg BSA/µg PBSF/SDF-1β.
Purity≥97% by SDS-PAGE
ContaminantsEndotoxin: ≤100 pg/µg PBSF/SDF-1β
Biological activityED₅₀ = 50-100 ng/ml as measured by its ability to chemoattract human T lymphocytes cultured in the presence of IL-2
SolubilityReconstitute to a concentration ≥50 µg/ml with sterile PBS containing as least 0.1% HSA or BSA.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long-term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C.
Toxicity Standard Handling
ReferencesBleul, C., et al. 1996. Nature 382, 829.
Oberlin, E., et al. 1996. Nature 382, 833.
Nagasawa, T., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 2305.
Tashiro, K., et al. 1993. Science 261, 600.