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2740-OP OmniPur® Bradford Method Protein Assay Kit - Calbiochem

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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2740-OP
  
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Overview

Replacement Information
Description
OverviewContains: Bradford Reagent, 0.15 M Sodium Chloride, and 0.5 mg/ml BSA Solution.

Specifications:
BSA Solution BSA Content: 0.45-0.55mg/ml
BSA Solution Protease: None detected
Bradford Reagent Pattern: Passes test
Bradford Reagent Protease: None detected
Sodium Chloride Solution Concentration: 130-170 mmol/l





Intended for laboratory and manufacturing use only. Not for drug, food, or household use.
Catalogue Number2740-OP
Brand Family Calbiochem®
References
Product Information
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Storage +2°C to +8°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
2740-OP 0

Documentation

OmniPur® Bradford Method Protein Assay Kit - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

OmniPur® Bradford Method Protein Assay Kit - Calbiochem Certificates of Analysis

TitleLot Number
2740-OP
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision22-September-2012 JSW
DescriptionThe Bradford method is a rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding.
Recommended reaction conditions

Materials Supplied
• 1 liter of Bradford reagent
• 1.5 ml of BSA
• 200 ml of Sodium Chloride solution

Protocol
1. Into 4 separate microcentrifuge tubes, aliquot 5, 10, 15 and 20 µl of 0.5 mg/ml BSA solution. Bring the volume of each to 100 µl with 0.15N NaCl.
2. Into 1 tube, aliquot 100 µl 0.15N NaCl. This will serve as a blank.
3. Add to each tube, 1 ml Bradford Reagent and vortex. Allow to stand at room temperature for 2 minutes.
4. Determine A 595 nm using a 1 ml microcuvette. Generate a standard curve by plotting absorbance at 595 nm versus protein concentration.
5. For the unknown sample, repeat steps 1-4 using the unknown in place of the BSA. Plot the A 595 nm and use the standard curve as a reference to determine the concentration of the unknown sample.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze