Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

482251 NIPP-1, His•Tag®, Bovine Thymus, Recombinant, E. coli - Calbiochem

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Nuclear Inhibitor of Protein Phosphatase-1

Primary Target: PP1
482251
  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
OverviewRecombinant, bovine NIPP-1 fused to a His•Tag® sequence and expressed in E. coli. Potent and specific inhibitor of protein phosphatase 1 (PP1; Ki = 1-10 pM) that can be used to distinguish PP1 from other major serine/threonine protein phosphatases including PP2A, PP2B, and PP2C. Also an ideal substrate for protein kinase A and casein kinase II. Thought to be involved in the targeting of PP1 to RNA-associated substrates and may also be involved in the dephosphorylation of transcription factors like CREB and the tumor suppressor Rb.

This product has been discontinued.
Catalogue Number482251
Brand Family Calbiochem®
SynonymsNuclear Inhibitor of Protein Phosphatase-1
References
ReferencesBeullens, M., et al. 1999. J. Biol. Chem. 274, 14053.
Jagiello, I., et al. 1997. J. Biol. Chem. 272, 22067.
Jagiello, I., et al. 1995. J. Biol. Chem. 270, 17257.
Van Eynde, A., et al. 1995. J. Biol. Chem. 270, 28068.
Van Eynde, A., et al. 1994. Biochem. J. 297, 447.
Beullens, M., et al. 1993. J. Biol. Chem. 268, 13172.
Beullens, M., et al. 1992. J. Biol. Chem. 267, 16538.
Product Information
ATP CompetitiveN
FormLiquid
FormulationIn 500 mM NaCl, 20 mM Tris, 500 µM benzamidine, 500 µM PMSF, 5 µM leupeptin, pH 7.5.
ReversibleN
Applications
Biological Information
Primary TargetPP1
Primary Target K<sub>i</sub>1-10 pM against protein phosphatase 1 (PP1)
Purity≥90% by SDS-PAGE
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Cell permeableN
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
482251 0

Documentation

NIPP-1, His•Tag®, Bovine Thymus, Recombinant, E. coli - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

NIPP-1, His•Tag®, Bovine Thymus, Recombinant, E. coli - Calbiochem Certificates of Analysis

TitleLot Number
482251

References

Reference overview
Beullens, M., et al. 1999. J. Biol. Chem. 274, 14053.
Jagiello, I., et al. 1997. J. Biol. Chem. 272, 22067.
Jagiello, I., et al. 1995. J. Biol. Chem. 270, 17257.
Van Eynde, A., et al. 1995. J. Biol. Chem. 270, 28068.
Van Eynde, A., et al. 1994. Biochem. J. 297, 447.
Beullens, M., et al. 1993. J. Biol. Chem. 268, 13172.
Beullens, M., et al. 1992. J. Biol. Chem. 267, 16538.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision13-November-2009 RFH
SynonymsNuclear Inhibitor of Protein Phosphatase-1
DescriptionRecombinant, bovine NIPP-1 fused to a His•Tag® sequence and expressed in E. coli. Potent and specific inhibitor or protein phosphatase 1 (PP1; Ki = 1-10 pM) that can be used to distinguish PP1 from other major serine/threonine protein phosphatases, including PP2A, PP2B, and PP2C. Reported to be an ideal substrate for protein kinase A (PKA) and casein kinase II (CKII). Believed to be involved in the targeting of PP1 to RNA-associated substrates. Also thought to be involved in the dephosphorylation of transcription factors like CREB and the tumor suppressor protein, Rb.
FormLiquid
FormulationIn 500 mM NaCl, 20 mM Tris, 500 µM benzamidine, 500 µM PMSF, 5 µM leupeptin, pH 7.5.
Concentration Label Please refer to vial label for lot-specific concentration
Purity≥90% by SDS-PAGE
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesBeullens, M., et al. 1999. J. Biol. Chem. 274, 14053.
Jagiello, I., et al. 1997. J. Biol. Chem. 272, 22067.
Jagiello, I., et al. 1995. J. Biol. Chem. 270, 17257.
Van Eynde, A., et al. 1995. J. Biol. Chem. 270, 28068.
Van Eynde, A., et al. 1994. Biochem. J. 297, 447.
Beullens, M., et al. 1993. J. Biol. Chem. 268, 13172.
Beullens, M., et al. 1992. J. Biol. Chem. 267, 16538.