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480025 NEMO-Binding Domain Binding Peptide, Cell-Permeable - Calbiochem

NEMO-Binding Domain Binding Peptide, Cell-Permeable, is an Antennapedia-NBD (wild type) fusion peptide that blocks the interaction of NEMO with the IKK (IκB)-kinase complex.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: DRQIKIWFQNRRMKWKKTALDWSWLQTE, NBD-Binding Peptide, Cell Permeable

Primary Target: cytokine-induced NF-κB activation and NF-κB-dependent gene expression
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Overview

Replacement Information

Key Spec Table

Empirical Formula
C₁₇₀H₂₅₉N₄₉O₄₂S
Description
Overview

This product has been discontinued.



A cell-permeable Antennapedia-NBD (wild type) fusion peptide that exhibits anti-inflammatory properties. Acts by inhibiting cytokine-induced NF-κB activation and NF-κB-dependent gene expression. Suggested to block the interaction of NEMO with the IKK (IκB)-kinase complex and thereby prevents NF-κB activation. Does not affect the TNF-α-induced phosphorylation of c-Jun and DNA binding of the transcription factor Oct-1.
Catalogue Number480025
Brand Family Calbiochem®
SynonymsDRQIKIWFQNRRMKWKKTALDWSWLQTE, NBD-Binding Peptide, Cell Permeable
References
ReferencesJimi, E., et al. 2004. Nat. Med., 10, 617.
Siegmund, D., et al. 2001. J. Biol. Chem. 276, 43708.
May, M.J., et al. 2000. Science 289, 1550.
Li, Q., et al. 1999. Science 284, 321.
Product Information
ATP CompetitiveN
FormWhite lyophilized solid
FormulationSupplied as a trifluoroacetate salt.
Hill FormulaC₁₇₀H₂₅₉N₄₉O₄₂S
Chemical formulaC₁₇₀H₂₅₉N₄₉O₄₂S
Hygroscopic Hygroscopic
ReversibleN
Quality LevelMQ100
Applications
ApplicationNEMO-Binding Domain Binding Peptide, Cell-Permeable, is an Antennapedia-NBD (wild type) fusion peptide that blocks the interaction of NEMO with the IKK (IκB)-kinase complex.
Biological Information
Primary Targetcytokine-induced NF-κB activation and NF-κB-dependent gene expression
Purity≥95% by HPLC
Physicochemical Information
Cell permeableY
Peptide SequenceH-Asp-Arg-Gln-Ile-Lys-Ile-Trp-Phe-Gln-Asn-Arg-Arg-Met-Lys-Trp-Lys-Lys-Thr-Ala-Leu-Asp-Trp-Ser-Trp-Leu-Gln-Thr-Glu-OH
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
480025 0

Documentation

NEMO-Binding Domain Binding Peptide, Cell-Permeable - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

NEMO-Binding Domain Binding Peptide, Cell-Permeable - Calbiochem Certificates of Analysis

TitleLot Number
480025

References

Reference overview
Jimi, E., et al. 2004. Nat. Med., 10, 617.
Siegmund, D., et al. 2001. J. Biol. Chem. 276, 43708.
May, M.J., et al. 2000. Science 289, 1550.
Li, Q., et al. 1999. Science 284, 321.

Brochure

Title
Pathways and Biomarkers of Toll-like Receptor (TLR) Signaling

Citations

Title
  • Soonkyu Chung, et al. (2005) Conjugated linoleic acid promotes human adipocyte insulin resistance through NF-κB-dependent cytokine production. Journal of Biological Chemistry 280, 38445-38456.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision06-May-2011 JSW
    SynonymsDRQIKIWFQNRRMKWKKTALDWSWLQTE, NBD-Binding Peptide, Cell Permeable
    DescriptionA cell-permeable Antennapedia-NBD (NEMO binding domain) (wild type) fusion peptide that exhibits anti-inflammatory activity in mouse model of acute inflammation. NBD is an amino terminal α-helical region of the NEMO (NF-κB essential modifier; IKKγ) associated with a carboxyl-terminal segment of IKKα and IKKβ. This peptide blocks the association of NEMO with the IKK complex and prevents NF-κB activation. NEMO is also reported to suppress the intrinsic basal activity of the IKK complex. Its anti-inflammatory properties are attributed to the inhibition of cytokine-induced NF-κB activation and NF-κB-dependent gene expression. Does neither affect the TNF-α-induced phosphorylation of c-Jun nor the DNA binding of Oct-1 transcription factor.
    FormWhite lyophilized solid
    FormulationSupplied as a trifluoroacetate salt.
    Intert gas (Yes/No) Packaged under inert gas
    Chemical formulaC₁₇₀H₂₅₉N₄₉O₄₂S
    Peptide SequenceH-Asp-Arg-Gln-Ile-Lys-Ile-Trp-Phe-Gln-Asn-Arg-Arg-Met-Lys-Trp-Lys-Lys-Thr-Ala-Leu-Asp-Trp-Ser-Trp-Leu-Gln-Thr-Glu-OH
    Purity≥95% by HPLC
    SolubilityDMSO (50 mg/ml). Note: This peptide will precipitate when added to tissue culture medium even at 100 µM concentrations. However, sufficient material will stay in the medium to permeate cells.
    Storage -20°C
    Hygroscopic
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
    Toxicity Standard Handling
    ReferencesJimi, E., et al. 2004. Nat. Med., 10, 617.
    Siegmund, D., et al. 2001. J. Biol. Chem. 276, 43708.
    May, M.J., et al. 2000. Science 289, 1550.
    Li, Q., et al. 1999. Science 284, 321.
    Citation
  • Soonkyu Chung, et al. (2005) Conjugated linoleic acid promotes human adipocyte insulin resistance through NF-κB-dependent cytokine production. Journal of Biological Chemistry 280, 38445-38456.
    		•