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475859 Mitochondrial Fusion Promoter, M1 - CAS 219315-22-7 - Calbiochem

A cell-permeable phenylhydrazone that restores mitochondrial tubular network formation in MEF lacking either of the two outer mitochondrial membrane.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: (E)-4-Chloro-2-(1-(2-(2,4,6-trichlorophenyl)hydrazono)ethyl)phenol

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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
219315-22-7C₁₄H₁₀Cl₄N₂O
Description
OverviewA cell-permeable phenylhydrazone that restores mitochondrial tubular network formation in MEF lacking either of the two outer mitochondrial membrane (OMM) mitofusins (EC50 = 5.3 and 4.42 µM, respectively, in Mfn1 or Mfn2 knockout MEF cells) or in MPP+-treated SH-SY5Y cells (5 µM 24 h), but not in MEF lacking both Mfn1/2 or the inner mitochondrial membrane (IMM) fusion mediator Opa1 (optic atrophy1). M1 (5 µM 24 h) is reported to boost the downregulated ATP5A & ATP5B protein level in Mfn1 or Mfn2 knockout MEF to the wild-type MEF level and ATPase inhibitor oligomycin (Cat. No. 495455) at 5 µM is shown to completely offset the mitochondrial fusion effect by 5 µM M1 in Mfn1 knockout MEF. Comparing to mdivi-1 (Cat. No. 475856), M1 exerts its effect via promoting fusion rather than inhibiting fission or division.
Catalogue Number475859
Brand Family Calbiochem®
Synonyms(E)-4-Chloro-2-(1-(2-(2,4,6-trichlorophenyl)hydrazono)ethyl)phenol
References
ReferencesWang, D., et al. 2012. Angew. Chem. Int. Ed. 51, 9302.
Product Information
CAS number219315-22-7
FormOff-white solid
Hill FormulaC₁₄H₁₀Cl₄N₂O
ReversibleY
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Purity≥98% by HPLC
Physicochemical Information
Cell permeableY
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
475859 0

Documentation

Mitochondrial Fusion Promoter, M1 - CAS 219315-22-7 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Mitochondrial Fusion Promoter, M1 - CAS 219315-22-7 - Calbiochem Certificates of Analysis

TitleLot Number
475859

References

Reference overview
Wang, D., et al. 2012. Angew. Chem. Int. Ed. 51, 9302.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision24-October-2012 JSW
Synonyms(E)-4-Chloro-2-(1-(2-(2,4,6-trichlorophenyl)hydrazono)ethyl)phenol
DescriptionA cell-permeable phenylhydrazone compound that restores mitochondrial tubular network formation from the fragmented mitochondria seen in MEF lacking either one of the two outer mitochondrial membrane (OMM) mitofusins (EC50 = 5.3 and 4.42 µM, respectively, in Mfn1 or Mfn2 knockout MEF cells) or MPP+-treated SH-SY5Y cells (5 µM 24 h), while displaying no effect on ER or lysosome morphology in Mfn1 knockout MEF. The effect of M1 is limited to enhancing weakened mitochondrial fusion machinery and M1 cannot by itself rebuild interconnected tubular mitochondria in MEF lacking both Mfn1/2 or the inner mitochondrial membrane (IMM) fusion mediator Opa1 (optic atrophy1). M1 (5 µM 24 h) is reported to boost the downregulated ATP5A and ATP5B protein level in either Mfn1 or Mfn2 knockout MEF to the wild-type MEF level and ATPase inhibitor oligomycin (Cat. No. 495455) at 5 µM is shown to completely offset the mitochondrial fusion effect by 5 µM M1 in Mfn1 knockout MEF. Both M1 and Z-VAD-FMK (Cat. No. 219007) are shown to protect SH-SY5Y against MPP+-induced neuronal toxicity and additive protection can be achieved via a combined treatment (62%, 73%, 77%, and 89% survival rate, respectively, with DMSO, 5 µM M1, 1 µM Z-VAD-FMK, and combined treatment). Comparing to mdivi-1 (Cat. No. 475856), M1 exerts its effect via promoting fusion rather than inhibiting fission or division.
FormOff-white solid
Intert gas (Yes/No) Packaged under inert gas
CAS number219315-22-7
Structure formulaStructure formula
Purity≥98% by HPLC
SolubilityDMSO (25 mg/ml)
Storage Protect from light
+2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Toxicity Standard Handling
ReferencesWang, D., et al. 2012. Angew. Chem. Int. Ed. 51, 9302.