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FCMAB191F Milli-Mark™ Anti-CD27-FITC Antibody, clone M-T271

This Milli-Mark Anti-CD27-FITC Antibody, clone M-T271 is validated for use in FC for the detection of CD27.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Immunoglobulins & Immunology UniProt Number: P26842
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FCMAB191F
100 tests  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFCMFITCMonoclonal Antibody
Description
Catalogue NumberFCMAB191F
Trade Name
  • Milli-Mark
DescriptionMilli-Mark™ Anti-CD27-FITC Antibody, clone M-T271
Alternate Names
  • CD27 antigen
  • CD27L receptor
  • T-cell activation antigen CD27
  • T14
  • Tumor necrosis factor receptor superfamily member 7
Background InformationCD27, a member of the nerve growth factor receptor (NGFR) superfamily, is a type I transmembrane protein consisting of a disulphide-linked 120 kDa homodimer (Bigler, 1988; Sugita, 1992; Morimoto, 1995). CD27 is expressed on mature thymocytes and on the majority of human peripheral blood T cells, on both CD4+ and CD8+ subsets. CD27 is also expressed on activated B cells and a fraction of resting natural killer (NK) cells (Maurer, 1990; Sugita, 1992). Among CD4+ T cells, CD27 is preferentially expressed on unprimed CD4+CD45RA+CD45R0- T cells, while primed CD4+CD45RA-CD45R0+ T cells express low levels of CD27 (Sugita, 1992). During activation, the expression of CD27 is increased on B cells and unprimed T cells, while primed T cells have a relatively low capacity for upregulation of CD27 (Morimoto, 1995; Hintzen, 1994). The activation is also accompanied by the appearance of a 32 kDa soluble form of CD27 (sCD27) found in supernatants of activated lymphocytes (Hintzen, 1994).
CD27 is believed to play a role in T cell activation, since the expression is upregulated during T cell activation (Bigler, 1988; Sugita, 1992; Morimoto, 1995; Maurer, 1990; Hintzen, 1994; Lens, 1995).
References
Product Information
FormatFITC
Control
  • PBMCs
PresentationPurified Mouse monoclonal IgG1 conjugated to FITC in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2.
Quality LevelMQ100
Applications
ApplicationThis Milli-Mark Anti-CD27-FITC Antibody, clone M-T271 is validated for use in FC for the detection of CD27.
Key Applications
  • Flow Cytometry
Biological Information
ImmunogenLeukemic peripheral blood T cells from a patient with chronic T cell leukemia (T-CLL)
CloneM-T271
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityAntibody recognizes Human CD27 antigen.
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Purification MethodProtein A Purfied
UniProt Number
Molecular Weight120 kDa (Bigler, 1988; Sugita, 1992; Morimoto, 1995)
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by flow cytometry using human PBMCs
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintained refrigerated at 2-8°C from date of receipt. Protected from light in undiluted aliquots for 6 months from date of receipt.
Packaging Information
Material Size100 tests
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
FCMAB191F 04053252289163

Documentation

Milli-Mark™ Anti-CD27-FITC Antibody, clone M-T271 SDS

Title

Safety Data Sheet (SDS) 

Milli-Mark™ Anti-CD27-FITC Antibody, clone M-T271 Certificates of Analysis

TitleLot Number
Milli-Mark Anti-CD27-FITC, clone M-T271 - 2007336 2007336
Milli-Mark Anti-CD27-FITC, clone M-T271 - NRG1761334 NRG1761334
Milli-Mark™ Anti-CD27-FITC, clone M-T271 - 2845085 2845085
Milli-Mark™ Anti-CD27-FITC, clone M-T271 - 3503850 3503850
Milli-Mark™ Anti-CD27-FITC, clone M-T271 - 4113040 4113040
Milli-Mark™ Anti-CD27-FITC, clone M-T271 -2859521 2859521
Milli-Mark™ Anti-CD27-FITC, clone M-T271 - 2464626 2464626

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Merck's Milli-Mark and FlowCellect brands include fluorescent conjugated antibodies and multiparameter kits for cellular analysis. Designed, optimized, and validated for flow.
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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies