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FCMAB418PE-I Milli-Mark™ Anti-CAR-PE Antibody, clone RmcB

Detect CAR using this Milli-Mark Anti-CAR-PE Antibody, clone RmcB validated for use in FC.

Milli-Mark™ Anti-CAR-PE Antibody, clone RmcB MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: ECM Proteins Gene Symbol: CXADR, CAR, hCAR, HCVADR, HCAR UniProt Number: P78310
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FCMAB418PE-I
100 tests  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFCMPhycoerythrinMonoclonal Antibody
Description
Catalogue NumberFCMAB418PE-I
Trade Name
  • Milli-Mark
DescriptionMilli-Mark™ Anti-CAR-PE Antibody, clone RmcB
Alternate Names
  • 46 kD coxsackievirus and adenovirus receptor (CAR) protein
  • CVB3 binding protein
  • CVB3-binding protein
  • Coxsackievirus B-adenovirus receptor
  • coxsackie virus B receptor
  • coxsackie virus and adenovirus receptor
Background InformationThe coxsackievirus and adenovirus receptor (CAR) mediates cell attachment and infection by coxsackie B viruses and by a number of adenoviruses. CAR also mediates homotypic intercellular interactions. In polarized epithelial cells, CAR is closely associated with the tight junction, where it contributes to the barrier to paracellular flow of solutes and macromolecules. CAR's biological roles are not well defined, but emerging evidence suggests that it may function during embryonic development and in regulating cell proliferation.
References
Product Information
FormatPhycoerythrin
Control
  • HT-29 cells
PresentationPurified mouse monoclonal IgG1 conjugated to PE in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Quality LevelMQ100
Applications
ApplicationDetect CAR using this Milli-Mark Anti-CAR-PE Antibody, clone RmcB validated for use in FC.
Key Applications
  • Flow Cytometry
Biological Information
ImmunogenNative human coxsackie virus-adenovirus receptor protein
CloneRmcB
HostMouse
SpecificityAntibody recognizes CAR.
IsotypeIgG1
Species Reactivity
  • Human
Species Reactivity NoteTested and passed on Human. Unconjugated version Cat. No. 05-644 is known to react with Mouse and Rat.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CXADR
  • CAR
  • hCAR
  • HCVADR
  • HCAR
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P78310 # Receptor for group B coxsackieviruses and subgroup C of adenoviruses (AD2 and AD5), susceptibility to infection is correlated to its level of expression and availability at the plasma membrane of cells.

SIZE: 365 amino acids; 40030 Da

SUBUNIT: Monomer. Probably homodimer formed by 2 molecules on adjacent cells. Interacts with LNX, MPDZ, BAIAP1, DLG4, PRKCABP, TJP1 and CTNNB1. Secreted isoform 3, isoform 4 and isoform 5 can interact with the extracellular domain of the receptor. Binds adenovirus 12 fiber protein. Isoform 5 but not isoform 3 binds to coxsackievirus.

SUBCELLULAR LOCATION: Isoform 1: Cell membrane; Single-pass type I membrane protein. Note=In epithelial cells localizes to the apical junction complex composed of tight and adherens junctions. In airway epithelial cells localized to basolateral membrane but not to apical surface. & Isoform 2: Cell membrane; Single-pass type I membrane protein. Note=In epithelial cells localizes to the apical junction complex composed of tight and adherens junctions. In airway epithelial cells localized to basolateral membrane but not to apical surface. & Isoform 3: Secreted. & Isoform 4: Secreted. & Isoform 5: Secreted.

TISSUE SPECIFICITY: Expressed in pancreas, brain, heart, small intestine, testis, prostate and at a lower level in liver and lung. Isoform 5 is ubiquitoulsy expressed while isoform 3 is expressed in heart, lung and pancreas. In skeletal muscle, isoform 1 is found at the neuromuscular junction and isoform 2 is found in blood vessels. In cardiac muscle, isoform 1 and isoform 2 are found at intercalated disks. In heart expressed in subendothelial layers of the vessel wall but not in the luminal endothelial surface. Expression is elevated in hearts with dilated cardiomyopathy.

DOMAIN: SwissProt: P78310 The Ig-like C2-type 1 domain probably mediates homodimerization and interaction with JAML. & The PDZ-binding motif mediates interaction with MPDZ and BAIAP1.

PTM: N-glycosylated (By similarity). & Palmitoylated on Cys-259 and/or Cys-260; required for proper localization to the plasma membrane.

SIMILARITY: Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Molecular Weight40 kDa Calculated. Uniprot indicates multiple isoforms ranging from 9 to 40 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by flow cytometry using HT-29 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
Packaging Information
Material Size100 tests
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
FCMAB418PE-I 04055977041521

Documentation

Milli-Mark™ Anti-CAR-PE Antibody, clone RmcB SDS

Title

Safety Data Sheet (SDS) 

Milli-Mark™ Anti-CAR-PE Antibody, clone RmcB Certificates of Analysis

TitleLot Number
Milli-Mark™ Anti-CAR-PE, -2646499 2646499
Milli-Mark™ Anti-CAR-PE, -2714452 2714452
Milli-Mark™ Anti-CAR-PE, -2806120 2806120
Milli-Mark™ Anti-CAR-PE, clone RmcB - 2519950 2519950
Milli-Mark™ Anti-CAR-PE, clone RmcB - 2563719 2563719
Milli-Mark™ Anti-CAR-PE, clone RmcB - 3415970 3415970
Milli-Mark™ Anti-CAR-PE, clone RmcB - 3510231 3510231
Milli-Mark™ Anti-CAR-PE, clone RmcB - 3724659 3724659
Milli-Mark™ Anti-CAR-PE, clone RmcB - 4150409 4150409
Milli-Mark™ Anti-CAR-PE, clone RmcB -2489986 2489986
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