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PF117 MT5-MMP, Catalytic Domain, His•Tag®, Human, Recombinant, E. coli

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: MMP-24, Catalytic Domain

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Overview

Replacement Information
Description
Overview

This product has been discontinued.



Recombinant, human pro-MT5-MMP fused to a His•Tag® sequence, expressed in E. coli and activated to yield the active catalytic domain. Two isoforms are produced, one with an N-terminal Tyr156, the other with an N-terminal Leu158. Useful for the study of the activation of progelatinase A and the degradation of extracellular matrix proteins. MT5-MMP is a member of the Zn2+- and Ca2+-dependent endopeptidases that functions in the degradation of extracellular matrix components such as gelatin, chondroitin sulfate, and dermatan sulfate. It has a tendency to be shed from cell surface membranes and appears to undergo rapid autocatalytic destruction into smaller fragments.
Catalogue NumberPF117
Brand Family Calbiochem®
SynonymsMMP-24, Catalytic Domain
References
ReferencesLlano, E., et al. 1999. Cancer Res. 59, 2570.
Pei, D. 1999. J. Biol. Chem. 274, 8925.
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme necessary to hydrolyze 1.0 μmol of the substrate MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg (Cat. No. 03-32-5032) per minute at 37°C, pH 7.5.
FormLiquid
FormulationIn 150 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, pH 7.5.
PreservativeNone
Quality LevelMQ100
Applications
Application NotesUseful for the investigation of the activation of progelatinase A and the degradation of extracellular matrix proteins.
Biological Information
Purity≥95% by SDS-PAGE
Specific Activity≥100 mU/mg protein
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
PF117 0

Documentation

MT5-MMP, Catalytic Domain, His•Tag®, Human, Recombinant, E. coli SDS

Title

Safety Data Sheet (SDS) 

MT5-MMP, Catalytic Domain, His•Tag®, Human, Recombinant, E. coli Certificates of Analysis

TitleLot Number
PF117

References

Reference overview
Llano, E., et al. 1999. Cancer Res. 59, 2570.
Pei, D. 1999. J. Biol. Chem. 274, 8925.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision10-July-2007 JSW
SynonymsMMP-24, Catalytic Domain
ApplicationUseful for the investigation of the activation of progelatinase A and the degradation of extracellular matrix proteins.
DescriptionRecombinant, human pro-MT5-MMP fused to a His•Tag® sequence, expressed in E. coli and activated to yield the active catalytic domain. Two molecular species are produced, one with an N-terminal Tyr156 and the other with an N-terminal Leu158. M.W. (longer sequence): 23,064. Appears as a doublet of ~22,000 by SDS-PAGE. Useful for the investigation of the activation of progelatinase A and the degradation of extracellular matrix proteins.

MT5-MMP (membrane-type matrix metalloproteinase-5, or MMP-24) is an ~74 kDa member of the Zn2+- and Ca2+-dependent endopeptidases that functions in the degradation of extracellular matrix components such as gelatin, chondroitin sulfate, and dermatan sulfate. MT5-MMP is expressed in adult brain, kidney, pancreas, and lung. Increased levels of MT5-MMP transcript have been found in astrocytomas, glioblastomas, and in pancreas and lung tumors. The enzyme has a tendency to be shed from cell surface membranes and appears to undergo rapid autocatalytic destruction into smaller fragments.
BackgroundMT5-MMP (membrane-type matrix metalloproteinase-5, or MMP-24) is an ~74 kDa member of the Zn2+- and Ca2+-dependent endopeptidases that functions in the degradation of extracellular matrix components such as gelatin, chondroitin sulfate, and dermatan sulfate. MT5-MMP is expressed in adult brain, kidney, pancreas, and lung. Increased levels of MT5-MMP transcript have been found in astrocytomas, glioblastomas, and in pancreas and lung tumors. The enzyme has a tendency to be shed from cell surface membranes and appears to undergo rapid autocatalytic destruction into smaller fragments.
FormLiquid
FormulationIn 150 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, pH 7.5.
Concentration Label Please refer to vial label for lot-specific concentration
Purity≥95% by SDS-PAGE
Specific activity≥100 mU/mg protein
Unit definitionOne unit is defined as the amount of enzyme necessary to hydrolyze 1.0 μmol of the substrate MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg (Cat. No. 03-32-5032) per minute at 37°C, pH 7.5.
PreservativeNone
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesLlano, E., et al. 1999. Cancer Res. 59, 2570.
Pei, D. 1999. J. Biol. Chem. 274, 8925.