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14-691M MRCKα Protein, active, 250 µg

Active, C-terminal 6His-tagged, recombinant, human MRCKα, amino acids 1-473. For use in Kinase Assays.

MRCKα Protein, active, 250 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-691M
250 µg  Also available in 10 μg size (cat#14-691) and in bulk (cat# 14-691-K).
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number14-691M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionMRCKα Protein, active, 250 µg
OverviewC-terminal 6His-tagged, recombinant, human MRCKα, amino acids 1-473.
References
Product Information
Quality LevelMQ100
Applications
ApplicationActive, C-terminal 6His-tagged, recombinant, human MRCKα, amino acids 1-473. For use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceMRCKα, amino acids 1-473, expressed by baculovirus in Sf21 cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a member of the Serine/Threonine protein kinase family. This kinase contains multiple functional domains. Its kinase domain is highly similar to that of the myotonic dystrophy protein kinase (DMPK). This kinase also contains a Rac interactive binding (CRIB) domain, and has been shown to bind CDC42. It may function as a CDC42 downstream effector mediating CDC42 induced peripheral actin formation, and promoting cytoskeletal reorganization. Multiple alternatively spliced transcript variants have been described, and the full-length nature of two of them has been reported.
Gene Symbol
  • CDC42BPA
  • DKFZp686L1738
  • PK428
  • DKFZp686P1738
  • KIAA0451
  • FLJ23347
  • MRCK
  • MRCKA
Protein TargetMRCKα
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyAGC
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q5VT25 # May act as a downstream effector of CDC42 in cytoskeletal reorganization. Contributes to the actomyosin contractility required for cell invasion, through the regulation of MYPT1 and thus MLC2 phosphorylation.
COFACTOR: Magnesium.
SIZE: 1732 amino acids; 197307 Da
SUBUNIT: Homodimer and homotetramer via the coiled coil regions. Interacts tightly with GTP-bound but not GDP-bound CDC42.
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Note=Displays a dispersed punctate distribution and concentrates along the cell periphery, especially at the leading edge and cell-cell junction. This concentration is PH-domain dependent (By similarity).
TISSUE SPECIFICITY: Abundant in the heart, brain, skeletal muscle, kidney, and pancreas, with little or no expression in the lung and liver.
SIMILARITY: SwissProt: Q5VT25 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 CNH domain. & Contains 1 CRIB domain. & Contains 1 PH domain. & Contains 1 phorbol-ester/DAG-type zinc finger. & Contains 1 protein kinase domain.
Molecular Weight55.3kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by phosphorylation of S6 Kinase/Rsk2 Substrate Peptide 2 (KKRNRTLTV)
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C from date of shipment
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-691) and in bulk (cat# 14-691-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
14-691M 04053252580932

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Alternative Packsize

Catalogue Number Description
14-691 MRCKα Protein, active, 10 µg

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Categories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases