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HSTC384-28K MILLIPLEX MAP 384-well Human High Sensitivity T Cell Panel - Immunology Multiplex Assay

This kit may be used for the analysis of all or any combination of the 384-well Human High Sensitivity T Cell panel analytes in serum, plasma, or tissue/cell culture supernatant. Because there are sev

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Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Inflammation & Immunology Research Sub-Category: Immune Response, Metabolic Disorders, Chronic Inflammatory Disease, Cardiovascular Disease, Neuroscience
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HSTC384-28K
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Overview

Key Spec Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
Fractalkine GM-CSF IFNγ IL-1β IL-2 IL-4 IL-5 IL-6 IL-7 IL-8 IL-10 IL-12 (p70) IL-13 IL-17A IL-21 IL-23 ITAC MIP-1α MIP-1β MIP-3α TNF-α HMplexLuminex xMAP
Description
Catalogue NumberHSTC384-28K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP 384-well Human High Sensitivity T Cell Panel - Immunology Multiplex Assay
OverviewEMD Millipore’s MILLIPLEX MAP 384-well Human High Sensitivity T Cell Panel is to be used for the simultaneous quantification of any or all of the above analytes in serum, plasma, or tissue/cell culture supernatant: Fractalkine/CX3CL1, GM-CSF, IFN-gamma, IL-1beta, IL-2, IL-4, IL-5, IL-6, IL-7, IL8/CXCL8, IL-10, IL-12 (p70), IL-13, IL17A/CTLA8, IL-21, IL-23, ITAC/EXCL11, MIP1alpha/CCL3, MIP1beta/CCL4, MIP-3alpha/CCL20 and TNFalpha.

MILLIPLEX® MAP offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. In addition to the assay characteristics listed in the protocol, other performance criteria evaluated during the validation process include: cross-reactivity, dilution linearity, kit stability and sample behavior (e.g. detectability and stability). Each panel and kit meets stringent manufacturing criteria to ensure batch-to-batch reproducibility.

Each MILLIPLEX® MAP panel and kit includes:
• Quality controls (QCs) provided to qualify assay performance

• Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency

• Optimized serum matrix to mimic native analyte environment

• Detection antibody cocktails designed to yield consistent analyte profiles within panel


EMD Millipore’s MILLIPLEX MAP 384-wellHuman High Sensitivity T Cell Panel is part of the most versatile system available for cytokine research. From our single to multiplex biomarker solutions, we partner with you to design, develop, analytically validate and build the most comprehensive library available for protein detection and quantitation.
Background InformationCytokines are immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses. A generic term, “cytokines” includes myokines (produced by muscle cells), lymphokines (produced by activated Th cells), interleukins (acting as mediators between T cells) and chemokines (responsible for T-cell migration). One of the regulatory mechanisms of the immune system, cytokines act at the recognition, activation, or effector phases of an immune response, modulating the development and functional activities of the subtypes of T cells, B cells and myeloid cells. Consequently, research involving cytokines plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, especially those responses that make up the inflammatory process.

Low levels of inflammation are involved in many clinical and sub-clinical disease states, such as autoimmune disease, cardiovascular disease, diabetes, neurological disorders and cancer. Measuring picogram levels of cytokines, therefore, is critical for understanding the pathogenesis of these diseases.

The 384-well Human High Sensitivity T Cell Panel is the 384-well version of the current 96-well Human High Sensitivity T Cell Panel, and it exceeds expectations in terms of throughput, sensitivity (see table) and sample detectability. Data obtained from the High Sensitivity T Cell Panel will correlate with data for the respective cytokines in the Human Cytokine/Chemokine Panels I, II and III, furthering your ability to measure specific cytokine response in both normal and disease states.
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Inter-assay: <20%
  • Intra-assay: <10%
Panel TypeMAGNETIC Cytokines/Chemokines
Quality LevelMQ200
Applications
ApplicationThis kit may be used for the analysis of all or any combination of the 384-well Human High Sensitivity T Cell panel analytes in serum, plasma, or tissue/cell culture supernatant. Because there are several distinctive steps, please read the entire protocol before running the assay.
Key Applications
  • Multiplexing
Application Notes• This is an overnight incubation.
• Because there are unique features concerning sample prep and assay set-up, please read the entire protocol before running the assay.
• This kit may be used for the analysis of all or any combination of the Human High Sensitivity T Cell panel analytes in serum, plasma or tissue/cell lysate and culture supernatant.
Biological Information
SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity
  • Human
Analytes Available
  • Fractalkine
  • GM-CSF
  • IFNγ
  • IL-1β
  • IL-2
  • IL-4
  • IL-5
  • IL-6
  • IL-7
  • IL-8
  • IL-10
  • IL-12 (p70)
  • IL-13
  • IL-17A
  • IL-21
  • IL-23
  • ITAC
  • MIP-1α
  • MIP-1β
  • MIP-3α
  • TNF-α
Cross ReactivityThere is no detectable cross-reactivity within the panel.
Accuracy
  • 79% – 108%
Physicochemical Information
Sensitivity
  • Please see kit protocol for individual assay sensitivities.
Linearity of DilutionPlease contact Technical Service for information.
Standard Curve Range
  • Fractalkine: 18.3 – 75,000 pg/mL
  • GM-CSF: 1.22 – 5,000 pg/mL
  • IFNγ: 0.61 – 2,500 pg/mL
  • IL-1β: 0.49 – 2,000 pg/mL
  • IL-2: 0.49 – 2,000 pg/mL
  • IL-4: 1.83 – 7,500 pg/mL
  • IL-5: 0.49 – 2,000 pg/mL
  • IL-6: 0.18 – 750 pg/mL
  • IL-7: 0.37 – 1,500 pg/mL
  • IL-8: 0.31 – 1,250 pg/mL
  • IL-10: 1.46 – 6,000 pg/mL
  • IL-12 (p70): 0.49 – 2,000 pg/mL
  • IL-13: 0.24 – 1,000 pg/mL
  • IL-17A: 0.73 – 3,000 pg/mL
  • IL-21: 0.24 – 1,000 pg/mL
  • IL-23: 7.93 – 32,500 pg/mL
  • ITAC: 1.46 – 6,000 pg/mL
  • MIP-1α: 0.31 – 1,250 pg/mL
  • MIP-1β: 0.92 – 3,750 pg/mL
  • MIP-3α: 0.61 – 2,500 pg/mL
  • TNFα: 0.43 – 1,750 pg/mL
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size384-well plate
Global Trade Item Number
Catalogue Number GTIN
HSTC384-28K 04054839156182