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48-669MAG MILLIPLEX® Early Apoptosis Magnetic Bead 7-plex Kit - Cell Signaling Multiplex Assay

Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation and total pathway proteins in tissue and cell lysate samples. Compare Multiplexing results to those of Western blotting.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Cell Signaling
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48-669MAG
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Overview

Replacement Information

Key Spec Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
Akt (pS473) BAD (Ser112) Bcl-2 (Ser70) p53 (Ser46) JNK (Thr183/Tyr185) Active Caspase-8 (Asp384) Active Caspase-9 (Asp315) HMplexLuminex xMAP
Description
Catalogue Number48-669MAG
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX® Early Apoptosis Magnetic Bead 7-plex Kit - Cell Signaling Multiplex Assay
OverviewApoptosis is an essential physiological process that plays a crucial role in normal development and homeostatic mechanisms of multicellular organisms. At least two major apoptotic pathways have been described in mammalian cells: the intrinsic pathway and the extrinsic pathway. Bcl-2 family members also play a pivotal role in the regulation of apoptosis as they reside upstream of irreversible cellular damage and focus much of their efforts at the level of mitochondria.

The MILLIPLEX® 7-plex Early Apoptosis Magnetic Bead Kit (Cat. No. 48-669MAG) is a premixed kit to be used for the simultaneous quantification of the following 7 analytes:

• Akt/PKB (Ser473)
• BAD (Ser112)
• Bcl-2 (Ser70)
• Active Caspase-8
• Active Caspase-9
• JNK/SAPK1 (Thr183/Tyr185)
• p53 (Ser46)
References
Product Information
Detection methodLuminex xMAP
HS Code3822 19 90
ConfigurationPremixed
Precision, %
  • Intra-Assay: < 15
  • Inter-Assay: < 20
Panel TypeCell Signaling
Quality LevelMQ200
Applications
ApplicationIntracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation and total pathway proteins in tissue and cell lysate samples. Compare Multiplexing results to those of Western blotting.
Key Applications
  • Multiplexing
Application Notes• An overnight (4°C) incubation is recommended for best results.
• This assay requires 25 µL diluted cell lysate per well.
• This kit must be run using Assay Buffer 2 (provided).
• The suggested working range of protein concentration for the assay is 1 to 25 µg of total protein/well (25 μL/well at 40 to 1,000 µg/mL).
Biological Information
SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity
  • Human
Analytes Available
  • Akt (pS473)
  • BAD (Ser112)
  • Bcl-2 (Ser70)
  • p53 (Ser46)
  • JNK (Thr183/Tyr185)
  • Active Caspase-8 (Asp384)
  • Active Caspase-9 (Asp315)
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Material Package96-well plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
48-669MAG 04053252820724

Documentation

Protocols

Title
Protocol- 48-669MAG

MILLIPLEX® Early Apoptosis Magnetic Bead 7-plex Kit - Cell Signaling Multiplex Assay SDS

Title

Safety Data Sheet (SDS) 

Brochure

Title
A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with Immunoassay Platform Solutions.
The Power of Biomarker Analysis

Technical Info

Title
96 well Template 2 Up
96 well Template Single

Data Sheet

Title
Full Service Custom-built Assays

Posters

Title
MILLIPLEX®MAP Cell Signaling Wall Chart