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14-173M Sigma-AldrichMAP Kinase 2/Erk2 Protein, active, mouse, 250 µg

Active, N-terminal GST-tagged, recombinant MAP Kinase 2, expressed in E. coli cells, activated using MEK1. For use in Kinase Assays.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

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Description
Catalogue Number	14-173M
Brand Family	Upstate
Trade Name	Upstate
Description	MAP Kinase 2/Erk2 Protein, active, mouse, 250 µg
Overview	N-terminal GST-tagged, recombinant MAP Kinase 2, expressed in E. coli cells; activated using MEK1 (Catalog #14-429).

References

Product Information
Presentation	50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 50% glycerol, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol.
Quality Level	MQ100

Applications
Application	Active, N-terminal GST-tagged, recombinant MAP Kinase 2, expressed in E. coli cells, activated using MEK1. For use in Kinase Assays.
Key Applications	Kinase Assay

Biological Information
Source	recombinant, mouse, full-length MAP Kinase 2
Specific Activity	For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number	NM_011949.3
Entrez Gene Summary	The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.
Gene Symbol	MAPK1 p40 PRKM2 MAPK2 PRKM1 p42-MAPK p38 p41 ERK ERK-2 ERT1 P42MAPK p41mapk ERK2
Protein Target	MAPK2
Purification Method	Glutathione agarose; Ni²⁺/NTA agarose
Target Sub-Family	CMGC
UniProt Number	P63085
UniProt Summary	FUNCTION: SwissProt: P63085 #Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The the MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. COFACTOR: Magnesium (By similarity). DOMAIN: The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain.
Molecular Weight	67.8kDa

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Routinely evaluated by phosphorylation myelin basic protein
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Store in undiluted aliquots at -20°C for up to one year from the date of receipt. Avoid repeatede freeze-thaw cycles.

Packaging Information
Material Size	250 µg
Material Package	Also available in 10 μg size (cat#14-173) and in bulk (cat# 14-173-K).

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
14-173M	04053252362149

Documentation

MAP Kinase 2/Erk2 Protein, active, mouse, 250 µg SDS

Title
Safety Data Sheet (SDS)

MAP Kinase 2/Erk2 Protein, active, mouse, 250 µg Certificates of Analysis

Title	Lot Number
MAP Kinase 2, active - D8KN012U-D	D8KN012U-D

Related Products & Applications

Product Families

Categories

Life Science Research > Proteins and Enzymes > Purified Kinases

Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases

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