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407611 Interleukin-1α, Human, Recombinant, E. coli

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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Overview

Replacement Information
Description
Overview

This product has been discontinued.



Recombinant, human

interleukin-1α expressed in E. coli. Potent stimulator of bone resorption and stimulator of nuclear phospholipase C. Induces nitric oxide synthase.
Catalogue Number407611
Brand Family Calbiochem®
References
ReferencesTsan, M.F., and White, J.E. 1994. Am. J. Physiol. 266, L316.
Kilbourn, R.G., et al. 1992. J. Natl. Cancer Inst. 84, 1008.
Dinarello, C.A., et al. 1991. Blood 77, 1627.
Casagli, M.C., et al. 1989. Biochem. Biophys. Res. Commun. 162, 357.
Symons, J.A., et al. 1987. In Lymphokines and Interferons, A Practical Approach (Clemens, M.J., et al. Eds.) Oxford IRL Press, p. 272.
March, C.J., et al. 1985. Nature 315, 641.
Kampschmidt, R.F. 1984. J. Leuk. Biol. 36, 341.
Product Information
FormLyophilized
FormulationLyophilized from sterile-filtered 20 mM Tris-HCl, 50 µg BSA/µg IL-1α, pH 8.3.
Quality LevelMQ100
Applications
Biological Information
Biological activityED₅₀ = 3-7 pg/ml as measured in a cell proliferation assay.
Purity≥97% by SDS-PAGE
Physicochemical Information
ContaminantsEndotoxin: ≤1.0 EU/µg IL-1α
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSNM9739900
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage ≤ -70°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
407611 0

Documentation

Interleukin-1α, Human, Recombinant, E. coli SDS

Title

Safety Data Sheet (SDS) 

Interleukin-1α, Human, Recombinant, E. coli Certificates of Analysis

TitleLot Number
407611

References

Reference overview
Tsan, M.F., and White, J.E. 1994. Am. J. Physiol. 266, L316.
Kilbourn, R.G., et al. 1992. J. Natl. Cancer Inst. 84, 1008.
Dinarello, C.A., et al. 1991. Blood 77, 1627.
Casagli, M.C., et al. 1989. Biochem. Biophys. Res. Commun. 162, 357.
Symons, J.A., et al. 1987. In Lymphokines and Interferons, A Practical Approach (Clemens, M.J., et al. Eds.) Oxford IRL Press, p. 272.
March, C.J., et al. 1985. Nature 315, 641.
Kampschmidt, R.F. 1984. J. Leuk. Biol. 36, 341.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision13-May-2008 RFH
DescriptionRecombinant, human interleukin-1α expressed in E. coli. A 159 amino acid polypeptide derived from mature IL-1α. Synthesized in vivo as a 271 amino acid residue (31 kDa) precursor that is subsequently cleaved into the mature polypeptide. Shows ~25% amino acid sequence identity with IL-1β, a distinct gene product sharing the same cell surface receptors as IL-1α. Produced by activated macrophages and many other cell types, the IL-1 proteins mediate a variety of effects on the differentiation and function of many cell types involved in inflammatory and immune responses. Potent stimulator of bone resorption and stimulator of nuclear phospholipase C. Induces nitric oxide synthase. Effects include stimulation of T-cell, B-cell, neutrophil, and fibroblast proliferation; induction of prostaglandin, leukotriene, and acute-phase hepatic protein synthesis; induction of proteinases in synovial fibroblasts and articular chondrocytes. IL-1 is also known to induce fever, slow-wave sleep, and bone resorption. 1 µg is equivalent to 1.6 x 105 WHO units.
FormLyophilized
FormulationLyophilized from sterile-filtered 20 mM Tris-HCl, 50 µg BSA/µg IL-1α, pH 8.3.
RTECSNM9739900
Purity≥97% by SDS-PAGE
ContaminantsEndotoxin: ≤1.0 EU/µg IL-1α
Biological activityED₅₀ = 3-7 pg/ml as measured in a cell proliferation assay.
SolubilityReconstitute with sterile PBS containing ≥0.1% HSA or BSA.
Storage ≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.
Toxicity Standard Handling
Merck USA index14, 4993
ReferencesTsan, M.F., and White, J.E. 1994. Am. J. Physiol. 266, L316.
Kilbourn, R.G., et al. 1992. J. Natl. Cancer Inst. 84, 1008.
Dinarello, C.A., et al. 1991. Blood 77, 1627.
Casagli, M.C., et al. 1989. Biochem. Biophys. Res. Commun. 162, 357.
Symons, J.A., et al. 1987. In Lymphokines and Interferons, A Practical Approach (Clemens, M.J., et al. Eds.) Oxford IRL Press, p. 272.
March, C.J., et al. 1985. Nature 315, 641.
Kampschmidt, R.F. 1984. J. Leuk. Biol. 36, 341.