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407252 Insulin-Like Growth Factor-I, Mouse, Recombinant, E. coli

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: mIGF-I

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Overview

Replacement Information

Key Spec Table

Purity
≥98% by SDS and HPLC
Description
Overview

This product has been discontinued.



Full-length, recombinant, mouse insulin-like growth factor I (IGF-I) expressed in E. coli. IGF-I regulates proliferation and survival signals through the Akt pathway. Mouse IGF-I shares 94% sequence identity with human IGF-I and exhibits cross-species reactivity. Useful in the study of cell proliferation and survival signals.
Catalogue Number407252
Brand Family Calbiochem®
SynonymsmIGF-I
References
ReferencesClemmons, D.R., et al. 2006. Curr. Opin. in Pharmacol. 6, 620.
Bell, G.I., et al. 1986. Nucleic Acids Res. 14, 7873.
Product Information
FormLyophilized
FormulationSterile-filtered prior to lyophilization. Contains no additives.
Quality LevelMQ100
Applications
Biological Information
Biological activityED₅₀: ≤2 ng/ml (corresponding to a specific activity of ≥1 x 10⁶ units/mg) as determined by a cell proliferation assay using FDC-P1 cells. For most in vitro applications IGF-I exerts is biological activity in the concentration range of 1-20 ng/ml.
Purity≥98% by SDS and HPLC
Physicochemical Information
ContaminantsEndotoxin: ≤1 ng/µg IGF-I
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. Avoid freeze/thaw cycles of solutions. Aliquots may be stored at 4°C for up to 1 week without loss of biological activity.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
407252 0

Documentation

Insulin-Like Growth Factor-I, Mouse, Recombinant, E. coli SDS

Title

Safety Data Sheet (SDS) 

Insulin-Like Growth Factor-I, Mouse, Recombinant, E. coli Certificates of Analysis

TitleLot Number
407252

References

Reference overview
Clemmons, D.R., et al. 2006. Curr. Opin. in Pharmacol. 6, 620.
Bell, G.I., et al. 1986. Nucleic Acids Res. 14, 7873.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision14-October-2008 JSW
SynonymsmIGF-I
DescriptionFull-length, recombinant, mouse insulin-like growth factor I (IGF-I) expressed in E. coli. IGF-I regulates proliferation and survival signals through the Akt pathway. Mouse IGF-I shares 94% sequence identity with human IGF-I and exhibits cross-species reactivity. Useful in the study of cell proliferation and survival signals.
FormLyophilized
FormulationSterile-filtered prior to lyophilization. Contains no additives.
Purity≥98% by SDS and HPLC
ContaminantsEndotoxin: ≤1 ng/µg IGF-I
Biological activityED₅₀: ≤2 ng/ml (corresponding to a specific activity of ≥1 x 10⁶ units/mg) as determined by a cell proliferation assay using FDC-P1 cells. For most in vitro applications IGF-I exerts is biological activity in the concentration range of 1-20 ng/ml.
SolubilityReconstitute with sterile H₂O to a final concentration of 0.1-1 mg/ml. Further dilute with aqueous buffer before use.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. Avoid freeze/thaw cycles of solutions. Aliquots may be stored at 4°C for up to 1 week without loss of biological activity.
Toxicity Standard Handling
ReferencesClemmons, D.R., et al. 2006. Curr. Opin. in Pharmacol. 6, 620.
Bell, G.I., et al. 1986. Nucleic Acids Res. 14, 7873.