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407240 Insulin-Like Growth Factor-I, Human, Recombinant, E. coli

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: rhIGF-I, Somatomedin-C

407240
  
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Overview

Replacement Information

Key Spec Table

Purity
≥97% by SDS-PAGE
Description
Overview

This product has been discontinued.





Recombinant, human insulin-like growth factor-I (70 amino acids) expressed in E. coli. Has about 40% homology to insulin and produces metabolic effects similar to insulin. Acts as an important local regulator of bone formation. Increases bone formation and bone resorption. Also inhibits etoposide-induced apoptosis in BALB/c 3T3 cells. Stimulates the activity of protein tyrosine kinases in both normal and diethylstilbestrol-treated hamster kidneys. Primarily involved in response of cells to growth hormones.
Catalogue Number407240
Brand Family Calbiochem®
SynonymsrhIGF-I, Somatomedin-C
References
ReferencesStabnov, L., et al. 2002. Bone 30, 909.
Devireddy, L.R., et al. 2001. Science 293, 829.
Chen, C.W., and Ray, D. 1995. Carcinogenesis 16, 1339.
Sell, C., et al. 1995. Cancer Res. 55, 303.
Amarani, S., et al. 1993. J. Bone Min. Res. 8, 157.
Mohan, S. 1993. Growth Regul. 3, 67.
Lowe, W.L. 1991. In Insulin-Like Growth Factors: Molecular and Cellular Aspects (LeRoith, D., ed.) CRC Press Inc. Boca Raton, FL. p. 49.
Rudd, B.T. 1991. Ann. Clin. Biochem. 28, 542.
Schofield, P.N. 1991. Acta Paediatr. Scand. 372, 83.
Werner, H., et al. 1991. In Molecular Biology and Physiology of Insulin and Insulin-Like Growth Factors (Raizada, M.K., and LeRoith, D., eds.) Plenum, NY. p. 263.
Humbel, R.E. 1984. In Hormonal Proteins and Peptides (Jin Li, C.H., ed.) Academic, NY. Vol. 12, p. 57.
Product Information
CAS number67763-96-6
FormLyophilized
Applications
Biological Information
Biological activityED₅₀ = 0.3-1.5 ng/ml as measured in a cell proliferation assay with MCF-7 human breast carcinoma cells.
Purity≥97% by SDS-PAGE
Physicochemical Information
ContaminantsEndotoxin: ≤1 EU/µg IGF-I
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSNM8905000
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage ≤ -70°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at -70°C. Dilute with buffer to desired pH just prior to use.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
407240 0

Documentation

Insulin-Like Growth Factor-I, Human, Recombinant, E. coli SDS

Title

Safety Data Sheet (SDS) 

Insulin-Like Growth Factor-I, Human, Recombinant, E. coli Certificates of Analysis

TitleLot Number
407240

References

Reference overview
Stabnov, L., et al. 2002. Bone 30, 909.
Devireddy, L.R., et al. 2001. Science 293, 829.
Chen, C.W., and Ray, D. 1995. Carcinogenesis 16, 1339.
Sell, C., et al. 1995. Cancer Res. 55, 303.
Amarani, S., et al. 1993. J. Bone Min. Res. 8, 157.
Mohan, S. 1993. Growth Regul. 3, 67.
Lowe, W.L. 1991. In Insulin-Like Growth Factors: Molecular and Cellular Aspects (LeRoith, D., ed.) CRC Press Inc. Boca Raton, FL. p. 49.
Rudd, B.T. 1991. Ann. Clin. Biochem. 28, 542.
Schofield, P.N. 1991. Acta Paediatr. Scand. 372, 83.
Werner, H., et al. 1991. In Molecular Biology and Physiology of Insulin and Insulin-Like Growth Factors (Raizada, M.K., and LeRoith, D., eds.) Plenum, NY. p. 263.
Humbel, R.E. 1984. In Hormonal Proteins and Peptides (Jin Li, C.H., ed.) Academic, NY. Vol. 12, p. 57.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision31-March-2008 JSW
SynonymsrhIGF-I, Somatomedin-C
DescriptionRecombinant, human insulin-like growth factor-I (70 amino acids) expressed in E. coli. Growth-promoting, single-chain polypeptide hormone that is structurally, functionally, and genetically related to insulin. Has about 40% homology to insulin and produces metabolic effects similar to insulin. Functionally, the IGFs share three activities with insulin: demonstrate insulin-like metabolic activity, induce sulfate uptake into cartilage proteoglycans, and induce mitotic activity in selected cell lines. Acts as an important local regulator of bone formation. Also inhibits etoposide-induced apoptosis in BALB/c 3T3 cells.
FormLyophilized
CAS number67763-96-6
RTECSNM8905000
Purity≥97% by SDS-PAGE
ContaminantsEndotoxin: ≤1 EU/µg IGF-I
Biological activityED₅₀ = 0.3-1.5 ng/ml as measured in a cell proliferation assay with MCF-7 human breast carcinoma cells.
SolubilityReconstitute to ≥0.1 mg/ml with sterile PBS containing 0.1% BSA or HSA.
Storage ≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at -70°C. Dilute with buffer to desired pH just prior to use.
Toxicity Standard Handling
Merck USA index14, 4986
ReferencesStabnov, L., et al. 2002. Bone 30, 909.
Devireddy, L.R., et al. 2001. Science 293, 829.
Chen, C.W., and Ray, D. 1995. Carcinogenesis 16, 1339.
Sell, C., et al. 1995. Cancer Res. 55, 303.
Amarani, S., et al. 1993. J. Bone Min. Res. 8, 157.
Mohan, S. 1993. Growth Regul. 3, 67.
Lowe, W.L. 1991. In Insulin-Like Growth Factors: Molecular and Cellular Aspects (LeRoith, D., ed.) CRC Press Inc. Boca Raton, FL. p. 49.
Rudd, B.T. 1991. Ann. Clin. Biochem. 28, 542.
Schofield, P.N. 1991. Acta Paediatr. Scand. 372, 83.
Werner, H., et al. 1991. In Molecular Biology and Physiology of Insulin and Insulin-Like Growth Factors (Raizada, M.K., and LeRoith, D., eds.) Plenum, NY. p. 263.
Humbel, R.E. 1984. In Hormonal Proteins and Peptides (Jin Li, C.H., ed.) Academic, NY. Vol. 12, p. 57.