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495609 InSolution™ Okadaic Acid, Prorocentrum sp. - Calbiochem

InSolution™ Okadaic Acid, Prorocentrum sp. CAS 78111-17-8, is a 250 µM solution of Okadaic Acid in DMSO. Potent inhibitor of protein phosphatase 1 (IC50 = 10-15 nM) and 2A (IC50 = 0.1 nM).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Primary Target: protein phosphatase
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Overview

Replacement Information

Key Spec Table

Empirical Formula
C₄₄H₆₈O₁₃
Description
Catalogue Number495609
Brand Family Calbiochem®
References
ReferencesGjertsen, B.T., et al. 1994. J. Cell Sci. 107, 3363.
Kiguchi, K., et al. 1994. Cell Growth Differentiation 5, 995.
Ohaka, Y., et al. 1993. Biochem. Biophys. Res. Commun. 197, 916.
Gopalakrishna, R., et al. 1992. Biochem. Biophys. Res. Commun. 189, 950.
Kreienbuhl, P., et al. 1992. Blood 80, 2911.
Nomura, M., et al. 1992. Biochemistry 31, 11915.
Song, Q., et al. 1992. J. Cell Physiol. 153, 550.
Tada, Y., et al. 1992. Immunopharmacol. 24, 17.
Cohen, P., et al. 1990. Trends Biochem. Sci. 15, 98.
Cohen, P. 1989. Annu. Rev. Biochem. 58, 453.
Cohen, P., and Cohen, P.T. 1989. J. Biol. Chem. 264, 21435.
Haystead, T.A., et al. 1989. Nature 337, 78.
Product Information
ATP CompetitiveN
FormLiquid
FormulationA 250 µM (25 µg/124 µl) solution of Okadaic Acid, (Cat. No. 495604) in DMSO.
Hill FormulaC₄₄H₆₈O₁₃
Chemical formulaC₄₄H₆₈O₁₃
Hygroscopic Hygroscopic
ReversibleN
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
ApplicationInSolution™ Okadaic Acid, Prorocentrum sp. CAS 78111-17-8, is a 250 µM solution of Okadaic Acid in DMSO. Potent inhibitor of protein phosphatase 1 (IC50 = 10-15 nM) and 2A (IC50 = 0.1 nM).
Biological Information
Primary Targetprotein phosphatase
Primary Target IC<sub>50</sub>10-15 nM against protein phosphatase 1; 0.1 nM against protein phosphatase 2A
Purity≥95% by HPLC
Physicochemical Information
Cell permeableN
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Toxic
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage -20°C
Protect from Light Protect from light
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
495609 0

Documentation

InSolution™ Okadaic Acid, Prorocentrum sp. - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

InSolution™ Okadaic Acid, Prorocentrum sp. - Calbiochem Certificates of Analysis

TitleLot Number
495609

References

Reference overview
Gjertsen, B.T., et al. 1994. J. Cell Sci. 107, 3363.
Kiguchi, K., et al. 1994. Cell Growth Differentiation 5, 995.
Ohaka, Y., et al. 1993. Biochem. Biophys. Res. Commun. 197, 916.
Gopalakrishna, R., et al. 1992. Biochem. Biophys. Res. Commun. 189, 950.
Kreienbuhl, P., et al. 1992. Blood 80, 2911.
Nomura, M., et al. 1992. Biochemistry 31, 11915.
Song, Q., et al. 1992. J. Cell Physiol. 153, 550.
Tada, Y., et al. 1992. Immunopharmacol. 24, 17.
Cohen, P., et al. 1990. Trends Biochem. Sci. 15, 98.
Cohen, P. 1989. Annu. Rev. Biochem. 58, 453.
Cohen, P., and Cohen, P.T. 1989. J. Biol. Chem. 264, 21435.
Haystead, T.A., et al. 1989. Nature 337, 78.

Citations

Title
  • Mike T. Lin, et al. (2005) Ca2+-activated K+ channel-associated phosphatase and kinase activities during development. American Journal of Physiology Heart and Circulatory Physiology 289, H414-H425.
  • Ramesh M. Ray, Sujoy bhattacharya and Leonard R. Johnson. (2005) Protein phosphatase 2A (PP2A) regulates apoptosis in IEC-6 cells. Journal of Biological Chemistry 280, 31091-31100.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision13-March-2009 RFH
    DescriptionAn ionophore-like polyether derivative of a C38 fatty acid compound isolated from dinoflagellates that have fed on the marine sponge Halinchrondria okadai. It is a potent non-comepetitive, reversible inhibitor of serine/threonine-specific protein phosphatases 1 (PP1, IC50 = 10-15 nM, rabbit skeletal muscle, catalytic subunit) and 2A (PP2A, IC50 = 0.1 nM, rabbit skeletal muscle, catalytic subunit). It has only a trivial effect on protein phosphatase 2B (IC50 = 5 µM), a Ca2+/calmodulin-dependent enzyme, while PP2C, a Mg2+ dependent enzyme, is unaffected. Okadaic acid has no significant effect on the activities of tyrosine phosphatases, alkaline phosphatase, acid phosphatase, and inositol trisphosphatase. It is also a non-phorbol ester-type tumor promoter on mouse skin. Useful for the study of protein phosphatases and of PP1/PP2A in cell extracts, as well as in intact cells. Induces apoptosis in human breast carcinoma (MB-231 and MCF-7) and in myeloid cells, but inhibits glucocorticoid-induced apoptosis in T-cell hybridomas. Has marked contractile effect on smooth muscles and heart muscles. Implicated as a causative agent of diarrhetic shellfish poisoning.
    FormLiquid
    FormulationA 250 µM (25 µg/124 µl) solution of Okadaic Acid, (Cat. No. 495604) in DMSO.
    Intert gas (Yes/No) Packaged under inert gas
    Chemical formulaC₄₄H₆₈O₁₃
    Structure formulaStructure formula
    Purity≥95% by HPLC
    Storage Protect from light
    -20°C
    Hygroscopic
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
    Toxicity Toxic
    Merck USA index14, 6819
    ReferencesGjertsen, B.T., et al. 1994. J. Cell Sci. 107, 3363.
    Kiguchi, K., et al. 1994. Cell Growth Differentiation 5, 995.
    Ohaka, Y., et al. 1993. Biochem. Biophys. Res. Commun. 197, 916.
    Gopalakrishna, R., et al. 1992. Biochem. Biophys. Res. Commun. 189, 950.
    Kreienbuhl, P., et al. 1992. Blood 80, 2911.
    Nomura, M., et al. 1992. Biochemistry 31, 11915.
    Song, Q., et al. 1992. J. Cell Physiol. 153, 550.
    Tada, Y., et al. 1992. Immunopharmacol. 24, 17.
    Cohen, P., et al. 1990. Trends Biochem. Sci. 15, 98.
    Cohen, P. 1989. Annu. Rev. Biochem. 58, 453.
    Cohen, P., and Cohen, P.T. 1989. J. Biol. Chem. 264, 21435.
    Haystead, T.A., et al. 1989. Nature 337, 78.
    Citation
  • Mike T. Lin, et al. (2005) Ca2+-activated K+ channel-associated phosphatase and kinase activities during development. American Journal of Physiology Heart and Circulatory Physiology 289, H414-H425.
  • Ramesh M. Ray, Sujoy bhattacharya and Leonard R. Johnson. (2005) Protein phosphatase 2A (PP2A) regulates apoptosis in IEC-6 cells. Journal of Biological Chemistry 280, 31091-31100.
    		•