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405210 ID-8 - CAS 147591-46-6 - Calbiochem

A cell-permeable indole compound that, when added to murine D3ES ESC (embryonic stem cell) cultures in the absence of serum and MEF (murine embryonic fibroblast) feeder cells, serves as a cheaper, chemical alternative to LIF

ID-8 - CAS 147591-46-6 - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: 1-(4-Methoxyphenyl)-2-methyl-3-nitro-indol-6-ol

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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
405210-10MG Glass bottle 10 mg
Description
OverviewA cell-permeable indole compound that, when added (10 µM) to murine D3ES ESC (embryonic stem cell) cultures in the absence of serum and MEF (murine embryonic fibroblast) feeder cells, serves as a cheaper, chemical alternative to LIF (1,000 units/ml) for expanding ESCs in their undifferentiated state. Long-term murine ESCs maintained under ID-8 are capable of differentiating and EBs (Embryoid Bodies) formation upon ID-8 removal. Also claimed to maintain human ESCs in their pluripotent state in the absence of MEF.
Catalogue Number405210
Brand Family Calbiochem®
Synonyms1-(4-Methoxyphenyl)-2-methyl-3-nitro-indol-6-ol
References
ReferencesMiyabayashi, T., et al. 2008. Biosci. Biotechnol. Biochem. 72, 1242.
Product Information
CAS number147591-46-6
FormYellow powder
Hill FormulaC₁₆H₁₄N₂O₄
Chemical formulaC₁₆H₁₄N₂O₄
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Purity≥95% by HPLC
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
405210-10MG 04055977211856

Documentation

ID-8 - CAS 147591-46-6 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

ID-8 - CAS 147591-46-6 - Calbiochem Certificates of Analysis

TitleLot Number
405210

References

Reference overview
Miyabayashi, T., et al. 2008. Biosci. Biotechnol. Biochem. 72, 1242.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision21-October-2011 RFH
Synonyms1-(4-Methoxyphenyl)-2-methyl-3-nitro-indol-6-ol
DescriptionA cell-permeable indole compound that, when added (10 µM) to murine D3ES ESC (embryonic stem cell) cultures in the absence of serum and MEF (murine embryonic fibroblast) feeder cells, serves as a cheaper, chemical alternative to LIF (1,000 units/ml) for expanding ESCs in their undifferentiated state. Long-term murine ESCs maintained under ID-8 are capable of differentiating and EBs (Embryoid Bodies) formation upon ID-8 removal. Also claimed to maintain human ESCs in their pluripotent state in the absence of MEF.
FormYellow powder
Intert gas (Yes/No) Packaged under inert gas
CAS number147591-46-6
Chemical formulaC₁₆H₁₄N₂O₄
Structure formulaStructure formula
Purity≥95% by HPLC
SolubilityDMSO (50 mg/ml)
Storage Protect from light
+2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Toxicity Standard Handling
ReferencesMiyabayashi, T., et al. 2008. Biosci. Biotechnol. Biochem. 72, 1242.