Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

S4040 Hybrisol® I Hybridization Solution, (50% Formamide, 6X SSC)

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
View Products on Sigmaaldrich.com
S4040
500 mL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberS4040
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionHybrisol® I Hybridization Solution, (50% Formamide, 6X SSC)
OverviewHybrisol® Hybridization Solutions are pre-made, ready-to-use solutions optimized for sensitive single and multiple gene copy detection using membrane-based hybridization techniques. They are designed to give maximum signal and clean background for hybridizations performed on nylon and nitrocellulose membranes. Hybrisol® Solution contains 10% Dextran Sulfate, 1% SDS, 50% Formamide, 6X SSC, Sheared DNA, and Modified Denhardt's Solution.
References
Product Information
PresentationLiquid Hybrisol I ® Solution contains 10% Dextran Sulfate, 1% SDS, 50% Formamide, 6X SSC, Sheared DNA, and Modified

Denhardt's Solution.
Quality LevelMQ100
Applications
Key Applications
  • Northern Blotting
  • Southern Blotting
Application NotesBefore using, preheat at 37°C to 45°C for 10 - 20 minutes to dissolve precipitants and mix thoroughly. Use 1 ml for every 15 cm2 membrane for prehybridization and hybridization. Prehybridize for 60 minutes at 42°C to 45°C. Hybridize overnight at 45°C to 52°C. Prehybridization and hybridization temperatures may have to be determined empirically. Refer to the Nomograph located under the "Protocols" link at S4040 product specific page at www.chemicon.com for hybridization temperatures with various stringencies guidelines.
Biological Information
Physicochemical Information
Dimensions
Volume500 mL
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at room temperature tightly sealed when not in use. Solution can be stored refrigerated however it should be warmed to at least room temperature prior to use dilution for best results. Precipitates may form when the solution is cooled.
Packaging Information
Material Size500 mL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
S4040 04053252466724

Documentation

Hybrisol® I Hybridization Solution, (50% Formamide, 6X SSC) SDS

Title

Safety Data Sheet (SDS) 

Hybrisol® I Hybridization Solution, (50% Formamide, 6X SSC) Certificates of Analysis

TitleLot Number
HYBRISOL#174; I HYBRIDIZATION SOLUTION - 2190623 2190623
HYBRISOL® I HYBRIDIZATION SOLUTION 2464589
HYBRISOL® I HYBRIDIZATION SOLUTION 3113113
HYBRISOL® I HYBRIDIZATION SOLUTION 3046760
HYBRISOL® I HYBRIDIZATION SOLUTION 3137090
HYBRISOL® I HYBRIDIZATION SOLUTION 3030300
HYBRISOL® I HYBRIDIZATION SOLUTION - 2388863 2388863
HYBRISOL® I HYBRIDIZATION SOLUTION - 2279542 2279542
HYBRISOL® I HYBRIDIZATION SOLUTION - 2905361 2905361
HYBRISOL® I HYBRIDIZATION SOLUTION - 3144501 3144501
Show more

References

Reference overviewPub Med ID
A general method for the isolation of RNA complementary to DNA.
BOLTON, E T and McCARTHY, B J
Proc. Natl. Acad. Sci. U.S.A., 48: 1390-7 (1962)  1962

13870855 13870855