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SCR131 Human iPSC Derived Neural Progenitor Kit

Human iPSC Derived Neural Progenitor Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Cell Differentiation, Stem Cell Culture, Cell Culture, Neurotoxicity, Neuroscience
SCR131
1x10⁶ cells and 500ml optimized expansion medium/Kit  
Purchase on Sigma-Aldrich

Overview

Key Spec Table

Key Applications
Diff, Cult, CULT, Neurotoxicity, Neuroscience
Description
Catalogue NumberSCR131
DescriptionHuman iPSC Derived Neural Progenitor Kit
Alternate Names
  • Neural Stem Cell
  • IPS NSC
Background InformationEMD Millipore’s Human iPSC Derived Neural Progenitor Kit contains human neural progenitor cells (NPCs) along with optimized medium for their expansion. Human neural progenitor cells (NPCs) are derived from induced pluripotent stem (iPS) cells generated using STEMCCA Cre-Excisable Constitutive Polycistronic (OKSM) lentivirus. The viral transgenes have been removed using cell permeable TAT-Cre recombinase (Cat. No. SCR508) with excision confirmed by quantitative PCR of genomic DNA extracted from human iPSC cells.

These hiPSC-derived NPCs proliferate as an adherent cell monolayer with greater than 80% expression of the appropriate neural stem cell markers, including Nestin and Sox-2. Cells are provided at passage 3 and can be expanded for a further three to five passages with ENStem-A Neural Expansion Medium (Cat. No. SCM004). Human NPCs can be further differentiated to terminal neurons by the use of the Human ES/iPS Neuronal Differentiation Medium (Cat. No. SCM111) or ENStem-A Neuronal Differentiation Medium (Cat. No. SCM017). Differentiation leads to greater than 80-90% of the cells expressing mostly neuronal markers, bIII-tubulin and MAP2.

Human iPSC-derived NPCs can be used for a variety of research applications including studies of neurotoxicity, co-culture applications and screening for molecules that induce or inhibit preferential differentiation to mature neurons.
Product Information
Components
  • 1) 1x106 viable Human iPSC Derived Neural Progenitors: (Catalog No. SCC035). Store in liquid nitrogen.
  • 2) 500 mL ENStem-A Neural Expansion Medium (Catalog No. SCM004). Store at -20c.
Quality LevelMQ100
Applications
Key Applications
  • Cell Differentiation
  • Stem Cell Culture
  • Cell Culture
  • Neurotoxicity
  • Neuroscience
Biological Information
Stem Cell Type
  • Neural Stem Cells
  • Induced Pluripotent Stem Cells
Product Usage Statements
Usage Statement
  • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsENStem-ATM Neural Expansion Medium (Cat. No. SCM004) should be stored at -20C upon receiving.

Human iPSC-Derived Neural Progenitors Cells (Cat. No. SCC035) should be stored in liquid nitrogen immediately after receiving. Temperature fluctuations will impair the recovery of healthy cells. Do not thaw the human cells until you are ready to do the assay of choice.
Packaging Information
Material Size1x10⁶ cells and 500ml optimized expansion medium/Kit
Global Trade Item Number
Catalogue Number GTIN
SCR131 04053252910395