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12-403 Histone H3 Peptide, biotin conjugate

This peptide corresponds to residues 1-21 of histone H3.

Histone H3 Peptide, biotin conjugate MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Immunoprecipitation UniProt Number: Q16695 Entrez Gene Number: NM_002107.3
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12-403
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Key Spec Table

Key ApplicationsEntrez Gene NumberUni Prot Number
IPNM_002107.3 Q16695
Description
Catalogue Number12-403
Brand Family Upstate
Trade Name
  • Upstate
DescriptionHistone H3 Peptide, biotin conjugate
OverviewThis peptide corresponds to residues 1-21 of histone H3
References
Product Information
PresentationLyophilized powder
Quality LevelMQ100
Applications
ApplicationThis peptide corresponds to residues 1-21 of histone H3.
Key Applications
  • Immunoprecipitation
Biological Information
Entrez Gene Number
Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family.
Gene Symbol
  • H3F3A
  • MGC87783
  • H3.3A
  • MGC87782
  • H3F3
  • H3.3B
  • H3F3B
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
SIZE: 136 amino acids; 15508 Da
SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
SUBCELLULAR LOCATION: Nucleus.
PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity).
SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated as a substrate for histone-modifying enzymes.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions2 years at -20°C
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
12-403 04053252625732

Documentation

Histone H3 Peptide, biotin conjugate SDS

Title

Safety Data Sheet (SDS) 

Histone H3 Peptide, biotin conjugate Certificates of Analysis

TitleLot Number
Histone H3 Peptide, biotin conjugate (residues 1-21) - 2398835 2398835
Histone H3 Peptide, biotin conjugate (residues 1-21) 3023035
Histone H3 Peptide, biotin conjugate (residues 1-21) 2972455
Histone H3 Peptide, biotin conjugate (residues 1-21) 2898471
Histone H3 Peptide, biotin conjugate (residues 1-21) 2887972
Histone H3 Peptide, biotin conjugate (residues 1-21) - 2262659 2262659
Histone H3 Peptide, biotin conjugate (residues 1-21) - 2266458 2266458
Histone H3 Peptide, biotin conjugate (residues 1-21) - 2315706 2315706
Histone H3 Peptide, biotin conjugate (residues 1-21) - DAM1424999 DAM1424999
Histone H3 Peptide, biotin conjugate - 20144 20144
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Categories

Life Science Research > Proteins and Enzymes > Substrate & Control Proteins > Control Histones