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SCC117 Histone H2B-GFP expressing HeLa Cell Line

The Histone H2B-GFP expressing HeLa cell line is a useful cell model for imaging and analysis of chromosome dynamics.

Histone H2B-GFP expressing HeLa Cell Line MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Cell Culture, Cell Based Assays
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SCC117
≥1X10⁶ cells/vial  
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Overview

Key Spec Table

Key Applications
CULT, Cell Based Assays
Description
Catalogue NumberSCC117
DescriptionHistone H2B-GFP expressing HeLa Cell Line
Alternate Names
  • Histone H2B
Background InformationChromatin, the higher order structure of DNA, proteins and RNA, constitutes the majority of the nucleus of eukaryotic cells. Changes in chromatin structure are the essence of many essential nuclear processes, including transcription, meiosis, mitosis, and apoptosis. The nucleosome represents the primary building block of chromatin and it comprises an octomer of four core histone proteins (H2A, H2B, H3 and H4). HeLa cells expressing green fluorescent protein fused histone H2B (H2B-GFP) have been widely used to visualize the dynamics of chromosomal architecture in living cells during various processes. In addition to studying normal mitosis, histone H2B-GFP has been utilized for imaging the distinctive clustering behavior of double minute chromosomes (DMs) in cancer cells during mitosis, which contributes to their asymmetric distribution to daughter cells. Monitoring histone H2B-GFP also permits continuous analysis of chromosomal degradation during apoptosis.

References:
1. Workman, J.L., and Kingston, R.E. (1998). Alteration of nucleosome structure as a mechanism of transcriptional regulation. Annu. Rev.Biochem. 67:545–579.
2. Kanda, T., et al. (1998) Histone–GFP fusion protein enables sensitive analysis of chromosome dynamics in living mammalian cells. Curr. Biol. 8: 377-385.
3. Yamamoto, N., et al. (2004) Cellular dynamics visualized in live cells in vitro and in vivo by differential dual-color nuclear-cytoplasmic fluorescent-protein expression. Cancer Res. 64: 4251-4256.
Product Information
Quality LevelMQ100
Applications
ApplicationThe Histone H2B-GFP expressing HeLa cell line is a useful cell model for imaging and analysis of chromosome dynamics.
Key Applications
  • Cell Culture
  • Cell Based Assays
Application NotesThis product is intended for sale and sold solely for internal non-commercial research use per the terms of the “Restricted Use Agreement” as detailed in the product documentation. For information regarding any other uses, please contact licensing@emdmillipore.com.
Biological Information
Cell Line Type
  • Cancer Cells
Product Usage Statements
Quality Assurance• Each vial contains ≥ 1X106 viable cells.
• Cells are tested by PCR and are negative for HPV-16, HPV-18, Hepatitis A, B, C and HIV-1 & 2 viruses.
• Cells are negative for mycoplasma contamination.
• Each lot of cells are genotyped by STR analysis to verify the unique identity of the cell line.
Usage Statement
  • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsHistone H2B-GFP expressing HeLa Cell Line should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Packaging Information
Material Size≥1X10⁶ cells/vial
Global Trade Item Number
Catalogue Number GTIN
SCC117 04054839135408