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377853 Hirudin, Hirudo medicinalis, Recombinant, Saccharomyces cerevisiae - CAS 8001-27-2 - Calbiochem

Hirudin, Hirudo medicinalis, Recombinant, Saccharomyces cerevisiae, CAS 8001-27-2, is an anticoagulant that inactivates thrombin by blocking substrate binding groups.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Primary Target: Has highly specific antithrombin properties
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Overview

Replacement Information

Key Spec Table

CAS #
8001-27-2
Description
OverviewHV1 form devoid of sulfate residue at Tyr63. Has highly specific antithrombin properties. Inactivates thrombin by blocking substrate binding groups.
Catalogue Number377853
Brand Family Calbiochem®
References
Product Information
CAS number8001-27-2
Unit of DefinitionOne unit is defined as the amount of hirudin that will neutralize 1 NIH unit of thrombin (fibrinogen assay) at 37°C, pH 7.5.
ATP CompetitiveN
FormLyophilized
ReversibleN
Quality LevelMQ100
Applications
Biological Information
Primary TargetHas highly specific antithrombin properties
Specific Activity≥10,000 antithrombin units (ATU)/mg protein
Physicochemical Information
Cell permeableN
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSMR8440000
Safety Information
R PhraseR: 20/21/22

Harmful by inhalation, in contact with skin and if swallowed.
S PhraseS: 36

Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Harmful
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
377853 0

Documentation

Hirudin, Hirudo medicinalis, Recombinant, Saccharomyces cerevisiae - CAS 8001-27-2 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Hirudin, Hirudo medicinalis, Recombinant, Saccharomyces cerevisiae - CAS 8001-27-2 - Calbiochem Certificates of Analysis

TitleLot Number
377853
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision12-May-2008 RFH
DescriptionHV1 form devoid of the sulfate residue at Tyr63. Exhibits highly specific antithrombin properties. Inactivates thrombin by blocking substrate binding groups.
FormLyophilized
Recommended reaction conditions
Protocol for the Assay of Hirudin Reagents: 1. 100 NIH units/ml thrombin 2. 9 mg/ml isotonic saline (0.9%) 3. 0.5% fibrinogen in isotonic saline 4. hirudin reconstituted with 1.0 ml of distilled H2O, isotonic saline, or other suitable buffer Assay: 1. Add 10-100 µl of the sample to be assayed to 200 µl of 0.5% fibrinogen solution and mix well. 2. Add 5 µl aliquots of a standardized thrombin solution (100 NIH units/ml) at one min intervals with mixing at 20°C. 3. The end-point is reached when a fibrin clot appears within one minute. Calculate the number of antithrombin units (ATU) of hirudin present in the solution. Note: This protocol is meant to serve only as a guideline. It may need to be modified for specific applications.
CAS number8001-27-2
RTECSMR8440000
Specific activity≥10,000 antithrombin units (ATU)/mg protein
Unit definitionOne unit is defined as the amount of hirudin that will neutralize 1 NIH unit of thrombin (fibrinogen assay) at 37°C, pH 7.5.
SolubilityH₂O. Reconstitute with 1 ml H₂O.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Harmful
Merck USA index14, 4718