Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

111955 Hemacolor® Rapid staining of blood smear

Solution 1: fixing solution

Hemacolor® Rapid staining of blood smear MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: MeOH, Hydroxymethane, Methyl alcohol, Carbinol

View Products on Sigmaaldrich.com
111955
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
1119552500 Glass bottle 2.5 l
Description
Catalogue Number111955
SynonymsMeOH, Hydroxymethane, Methyl alcohol, Carbinol
DescriptionHemacolor® Rapid staining of blood smear
OverviewHemacolor® Rapid staining of blood smear - Solution 1: fixing solution, is a ready-to-use solution used for human-medical cell diagnosis and serves the fixing of native blood and bone-marrow smears of human origin in hematological and clinico-cytological specimen materials, for example smears of whole blood and bone marrow. The staining with Hemacolor® yields a staining result that corresponds to the Pappenheim stain, with predominantly magenta stained nuclei. This is based on the molecular interaction of the Eosin Y dye and a complex of Azur B with DNA. Both dyes assemble to an Eosin Y - Azur B-DNA complex and the intensity of the resulting stain depends on the content of Azur B and the ratio of Azur B : Eosin Y. Furthermore, the resulting stain can vary depending on the influence of fixation, staining times, pH-value of the solutions or buffer substances. By using pH 7.2 buffered solutions with the Hemacolor® Hemacolor® Rapid staining of blood smear solutions, a high stability of the stain and clean, precipitation-free staining results can be guaranteed.
The package is sufficient for 1250 - 5000 applications. The buffer tablets acc. to Weise pH 7.2 (Cat no. 109468) are also available in larger pack sizes, thus, they can be useful for higher demands. The three solutions needed for staining are all available individually as:

Hemacolor® Rapid staining of blood smear solution 1, fixing solution (Cat no. 111955)
Hemacolor® Rapid staining of blood smear solution 2, colour reagent red (Cat no. 111956)
Hemacolor® Rapid staining of blood smear solution 3, colour reagent blue (Cat no. 111957)

or they can be purchased as staining sets:

Hemacolor® Rapid staining of blood smear (Cat. no. 111674) 3 x 100 ml
Hemacolor® Rapid staining of blood smear (Cat. no. 111661) 3 x 500 ml

For more details, please see instructions for use (IFU). This product is registered as IVD and CE marked.
References
Product Information
CAS number67-56-1
EC index number603-001-00-X
EC number200-659-6
Hill FormulaCH₄O
Chemical formulaCH₃OH
Molar Mass32.04 g/mol
HS Code2905 11 00
Structure formula ImageStructure formula Image
Quality LevelMQ400
Applications
ApplicationSolution 1: fixing solution
Biological Information
Physicochemical Information
Boiling point64.5 °C (1013 hPa)
Density0.792 g/cm3 (20 °C)
Evaporation number1.9
Explosion limit5.5 - 44 %(V)
Flash point9.7 °C
Ignition temperature420 °C DIN 51794
Melting Point-97.8 °C
Vapor pressure128 hPa (20 °C)
Viscosity kinematic0.54 - 0.59 mm2/s (20 °C) no data available
Dimensions
Materials Information
Toxicological Information
LD 50 oralLD50 Rat 5628 mg/kg
LD 50 dermalLD50 Rabbit 17100 mg/kg
Safety Information according to GHS
Hazard Pictogram(s) Hazard Pictogram(s)
Hazard Pictogram(s)
Hazard Pictogram(s)
Hazard Statement(s)H225: Highly flammable liquid and vapour.
H301 + H311 + H331: Toxic if swallowed, in contact with skin or if inhaled.
H370: Causes damage to organs.
Precautionary Statement(s)P210: Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
P233: Keep container tightly closed.
P280: Wear protective gloves/ protective clothing/ eye protection/ face protection/ hearing protection.
P301 + P310: IF SWALLOWED: Immediately call a POISON CENTER/doctor.
P303 + P361 + P353: IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water.
P304 + P340 + P311: IF INHALED: Remove person to fresh air and keep comfortable for breathing. Call a POISON CENTER/doctor.
P403 + P233: Store in a well-ventilated place. Keep container tightly closed.
Signal WordDanger
RTECSPC1400000
Storage class3 Flammable liquids
WGKWGK 2 obviously hazardous to water
Disposal1
Strongly contaminated halogen-free organic solvents: container A.
Safety Information
Categories of dangerhighly flammable, toxic
Product Usage Statements
Storage and Shipping Information
StorageStore at +15°C to +25°C.
Packaging Information
Transport Information
Declaration (railroad and road) ADR, RIDUN 1230 , 3 (6.1), II
Declaration (transport by air) IATA-DGRUN 1230 , 3 (6.1), II
Declaration (transport by sea) IMDG-CodeUN 1230 , 3 (6.1), II
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
1119552500 04022536137629

Related Products & Applications

Related Products

Catalogue Number Description
111674 Hemacolor® Rapid staining of blood smear

Accessories (Primary)

Catalogue Number Description
109468 Buffer tablets pH 7.2
111956 Hemacolor® Rapid staining of blood smear
111957 Hemacolor® Rapid staining of blood smear

Accessories (Secondary)

Catalogue Number Description
111956 Hemacolor® Rapid staining of blood smear
111957 Hemacolor® Rapid staining of blood smear

Related Products By: Application Facete

Fixation
Blood Staining

Related Products By: Brand Facete

Hemacolor®

Categories

IVD/OEM Materials & Reagents > Microscopy > Hematology > Rapid Stainings, Hemacolor®
IVD/OEM Materials & Reagents > Microscopy > Cytology > Cytological Staining Reagents
IVD/OEM Materials & Reagents > Microscopy > Hematology > Hematological Staining Solutions