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71493 HRV 3C Protease

Highly efficient fusion tag removal at low temperature

HRV 3C Protease MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
71493
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
71493-10000UN Glass bottle 10000 un
71493-3 Glass bottle 500 u
Description
OverviewRecombinant type 14 3C protease from human rhinovirus (HRV 3C) is a recombinant restriction-grade protease. HRV 3C is a highly purified recombinant 6XHis-fusion protein, that recognizes the same cleavage site as the native enzyme: LeuGluValLeuPheGln↓GlyPro. The small, 22-kDa size of the protease, optimal activity at 4°C, high specificity, and His•Tag® fusion make HRV 3C protease an ideal choice for rapid removal of fusion tags. The pET expression vectors pET-47b(+) through pET-50b(+) incorporate the HRV 3C protease cleavage site in combinations with His•Tag, S•Tag™, thioredoxin (Trx•Tag™), glutathione-S-transferase (GST•Tag™), and NusA (Nus•Tag™) sequences. The combination of pET-47b(+) to pET-50b(+) vectors for expression, HRV 3C protease for fusion tag cleavage, and Ni-NTA His•Bind® metal affinity chromatography for protein purification as well as protease and fusion tag removal allows production of recombinant proteins free of vector-encoded sequences. Other expression vectors, including the pET-52b(+) vector, the InsectDirect™ pIEx™-9 vector, and the multisystem pTriEx™-6 vector, all incorporate the HRV 3C protease cleavage site in combination with the Strep•Tag® II and His•Tag sequences. Supplied as: 1.8-3.0 U/µl in 50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 1 mM EDTA, 0.5 mM THP, and 50% glycerol.
Catalogue Number71493
Brand Family Novagen®
References
Product Information
Activity1.8-3.0 U/µl
Unit of DefinitionOne unit will cleave >95% of 100 µg His•Tag fusion control protein in 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 at 4°C for 16 h.
•500 UHRV 3C Protease
•10 µgHRV 3C Cleavage Control Protein
•10 ml10X HRV 3C Cleavage Buffer
FormulationIn 50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 1 mM EDTA, 0.5 mM THP, and 50% glycerol.
Quality LevelMQ400
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
71493-10000UN 04055977270259
71493-3 07790788053277

Documentation

HRV 3C Protease Certificates of Analysis

TitleLot Number
71493

Brochure

Title
Bulk Product Guide

Citations

Title
  • Yanqiu Yuan, et al. (2007) Crystal structure of a peptidoglycan glycosyltransferase suggests a model for processive glycan chain synthesis. Procedings of the National Academy of Science 104, 5348-5353.
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    User Protocols

    Title
    TB420