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69453 HMS174(DE3) Competent Cells - Novagen

HMS174 strains provide high transformation efficiencies and the recA mutation in a K-12 background. Strain may stabilize certain target genes whose products may cause the loss of the DE3 prophage.

HMS174(DE3) Competent Cells - Novagen MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
69453-3 Plastic ampoule 0.4 ml
69453-4 Glass bottle 1 ml
Description
OverviewHMS174 strains provide the recA mutation in a K-12 background. Like BLR, these strains may stabilize certain target genes whose products may cause the loss of the DE3 prophage.

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
Genotype: F- recA1 hsdR(rK12- mK12+) (DE3) (Rif R)


This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.




This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
Catalogue Number69453
Brand Family Novagen®
References
Product Information
Components
Guaranteed efficiencyGuaranteed efficiency: > 5 x 10⁶ cfu/µg
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
69453-3 04055977274394
69453-4 04055977274400

Documentation

HMS174(DE3) Competent Cells - Novagen SDS

Title

Safety Data Sheet (SDS) 

HMS174(DE3) Competent Cells - Novagen Certificates of Analysis

TitleLot Number
69453

Brochure

Title
Competent Cell Brochure
The Complete Molecular Biology Toolkit - Expert workflow solutions from DNA cloning to protein expression

Citations

Title
  • Monika Walter, et al. (2008) Structure of the receptor-binding protein of bacteriophage Det7: a podoviral tail spike in a myovirus. Journal of Virology 82, 2265-2273.
  • Ishara Azmi, et al. (2006) Recycling of ESCRTs by the AAA-ATPase Vps4 is regulated by a conserved VSL region in Vta1. Journal of Cell Biology 172, 705-717.
  • Felice Arcuri, et al. (2005) The translationally controlled tumor protein is a novel calcium binding protein of the human placenta andregulates calcium handling in trophoblast cells. Biology of Reproduction 73, 745-751.
  • Helen N. Murphy, et al. (2005) The OtsAB pathway is essential for trehalose biosynthesis in Mycobacterium tuberculosis. Journal of Biological Chemistry 280, 14524-14529.
  • Ken Okada and Toshiharu Hase. (2005) Cyanobacterial non-mevalonate pathway: (E)-4-hydroxy-3-methylbut-2-enyl diphosphate synthase interacts with ferredoxin in Thermosynechococcus elongatus BP-1. Journal of Biological Chemistry 280, 60672-20679.
  • James B. Thoden and Hazel M. Holden. (2005) The molecular architecture of galactose mutarotase/UDP-galactose 4-epimerase from Saccharomyces cerevisiae. Journal of Biological Chemistry 280, 21900-21907.
  • Stacey A. L. Tom-Yew, et al. (2005) Anion-independent iron coordination by the Campylobacter jejuni ferric binding protein. Journal of Biological Chemistry 280, 9283-9290.
  • Sharon Yagur-Kroll and Orna Amster-Choder. (2005) Dynamic membrane topology of the E. coli -glucoside transporter β-glucosidase transporter BglF. Journal of Biological Chemistry 280, 19306-19318.
  • Bin Zhao, et al. (2005) Binding of two flaviolin substrate molecules, oxidative coupling, and crystal structure of Streptomyces coelicolor A3(2) cytochrome P450 158A2. Journal of Biological Chemistry 280, 11599-11607.
  • B. Huang, et al. (1996) NMR structure and mutagenesis of the Fas (APO-1/CD95) death domain. 384, 638-641.
  • Ramavedi Gudi, et al. (1995) Diversity of the pyruvate dehydrogenase kinase gene family in humans. Journal of Biological Chemistry 270, 28989-28994.
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    User Protocols

    Title
    TB009 Competent Cells
    TB053 Academic and Non-profit Laboratory Assurance Letter