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70532 GST•Tag™ Assay Kit

Quantitative assay of GST•Tag fusion proteins

GST•Tag™ Assay Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
70532
  
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Overview

Replacement Information
Description
Overview

This product has been discontinued.





The GST•Tag™ Assay Kit is designed to perform quantitative colorimetric enzymatic assays of glutathione-S-transferase (GST) fusion proteins (Habig 1974). The kit is useful for the quantification of GST activity in crude samples or purified fractions. The suitability of this assay for crude samples allows expression conditions to be evaluated and rapidly optimized by comparing GST activity levels. The GST activity assay is simple to perform using the supplied 1-chloro-2,4-dinitrobenzene (CDNB) substrate. A sample is combined with CDNB substrate in the supplied reaction buffer and the absorbance of the reaction is monitored at 340 nm. The rate of change in A340 is proportionate to the amount of GST activity present in the sample. The assay has sufficient sensitivity to detect as little as eight pmol of functional GST, which corresponds to approximately 250 ng of unfused GST.
Catalogue Number70532
Brand Family Novagen®
References
ReferencesHabig, W.H., et al. 1974. J. Biol. Chem. 249, 7130.
Product Information
•2 x 5 ml10X GST•Tag Assay Buffer
•1.2 ml100 mM CDNB
•1.2 ml100 mM Reduced Glutathione
•50 µgGST•Tag Standard
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 11-23/24/25-33-36/37/38-50/53

Highly flammable.
Toxic by inhalation, in contact with skin and if swallowed.
Danger of cumulative effects.
Irritating to eyes, respiratory system and skin.
Very toxic to aquatic organisms; may cause long-term adverse effects in the aquatic environment.
S PhraseS: 7-16-26-36/37-45-28-60-61

Keep container tightly closed.
Keep away from sources of ignition - No Smoking.
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing and gloves.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).

This material and its container must be disposed of as hazardous waste.
Avoid release to the environment. Refer to special instructions/safety data sheet.
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Multiple Toxicity Values, refer to MSDS
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
70532 0

Documentation

GST•Tag™ Assay Kit Certificates of Analysis

TitleLot Number
70532

References

Reference overview
Habig, W.H., et al. 1974. J. Biol. Chem. 249, 7130.

Citations

Title
  • John K. Mensah, et al. (2004) Functional analysis of a mutation in PAX9 associated with familial tooth agenesis in humans. Journal of Biological Chemistry 279, 5924-5933.
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    User Protocols

    Title
    TB236 GST•Tag™ Assay Kit