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69036 Factor Xa, Restriction Grade, Bovine Plasma

69036
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Overview

Products

Catalogue NumberPackaging Qty/Pack
69036-2000UG Glass bottle 2000 μg
69036-3 Plastic ampoule 100 μg
69036-500UG Glass bottle 500 μg
Description
OverviewRestriction Grade Factor Xa is a highly purified preparation isolated from bovine plasma and activated with Russell's viper venom. This preparation is purified to near homogeneity and shows no secondary cleavage from contaminating proteases. The preparation is also functionally tested for activity with fusion proteins. Like enterokinase, Factor Xa cleaves at the C-terminal side of the recognition sequence (IleGluGlyArg↓) and can therefore be used for removing all vector-encoded sequences from appropriately designed constructs.

Specific Activity: ≥150 IU/mg

One IU is defined as the amount of enzyme that will release 1 µmole of p-nitroaniline per minute using methanesulfonyl-D-leucyl-glycyl-arginyl-paranitroanilide at 37°C, pH 8.4.

One µg of enzyme cleaves 50 µg Cleavage Control Protein to >95% completion in 16 hr at 25°C in a buffer containing 50 mM Tris-HCl pH 8.0, 100 mM NaCl, and 5 mM CaCl2.
Catalogue Number69036
Brand Family Novagen®
Product Information
100 µgRestriction Grade Factor Xa
2 mlFactor Xa Dilution/Storage Buffer
1 ml10X Factor Xa Cleavage Buffer
10 µgCleavage Control Protein
Quality LevelMQ100
Biological Information
Biological activityOne microgram of enzyme cleaves 50 µg Xa Cleavage Control Protein to >95% completion in 16 hours at 25°C in a buffer containing 50 mM Tris-HCl pH 8.0, 100 mM NaCl, and 5 mM CaCl₂.
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Global Trade Item Number
Catalogue Number GTIN
69036-2000UG 04055977259605
69036-3 04055977259636
69036-500UG 04055977259681