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330655 Estrogen Receptorα, Human, Recombinant, S. frugiperda

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: ER-α

330655
  
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Overview

Description
Overview

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Recombinant, human estrogen receptor-α expressed in S. frugiperda insect cells. A hormone-inducible transcription factor that can positively or negatively regulate expression of many genes involved in tissue growth and differentiation. ER-α is essential for induction of the oxytocin receptor by estrogen. Activated ER-α is involved in the modulation of transcription triggered by AP-1. Provided in a soluble, functionally-active form. Binds to estradiol (Kd = 300 pM) and to a fluorescein-labeled estrogen response element (ERE; Kd = 10 nM). Suitable for in vitro transcription assays.
Catalogue Number330655
Brand Family Calbiochem®
SynonymsER-α
References
ReferencesTeyssier, C., et al. 2001. J. Biol. Chem. 276, 36361.
Young, L.T., et al. 1998. NeuroReport 9, 933.
Tzukeman, M.T., et al. 1994. Mol. Endocrinol. 8, 21.
Beekman, J.M., et al. 1993. Mol. Endocrinol. 7, 1266.
Obourn, J.D., et al. 1993. Biochemistry 32, 6229.
Product Information
ActivityBinds ≥ 960 pmol/[³H]-estradiol/ml
FormLiquid
FormulationIn 500 mM KCl, 50 mM Tris, 2 mM DTT, 1 mM EDTA, 1 mM sodium vanadate, 10% glycerol, 0.02% NaN₃, pH 7.5.
Biological Information
Purity≥80% by SDS-PAGE
Specific ActivityBinds ≥ 5,000 pmol [³H]-estradiol/mg protein
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C). DO NOT VORTEX.
Global Trade Item Number
Catalogue Number GTIN
330655 0